外周感觉和交感神经节中阿片样物质受体样（ORL1）受体mRNA剪接变体的存在。

Presence of opioid receptor-like (ORL1) receptor mRNA splice variants in peripheral sensory and sympathetic neuronal ganglia.

作者信息

Xie G X, Meuser T, Pietruck C, Sharma M, Palmer P P

机构信息

Department of Anesthesiology & Perioperative Care, University of California, San Francisco, 94143-0464, USA.

出版信息

Life Sci. 1999;64(22):2029-37. doi: 10.1016/s0024-3205(99)00150-2.

DOI:10.1016/s0024-3205(99)00150-2

PMID:10374928

Abstract

The expression of ORL1 receptor mRNA splice variants is determined in peripheral sensory and sympathetic ganglia and compared to mRNA expression for the three classic opioid receptor subtypes (mu, delta, and kappa) using the method of reverse transcription-polymerase chain reaction. ORL1, mu, delta and kappa receptor subtype mRNAs are present in human dorsal root ganglia (DRG) and trigeminal ganglia and rat DRG. ORL1, mu and delta receptor subtype mRNAs are present in rat superior cervical ganglia and only ORL1 and delta receptor mRNAs are present in rat lumbar sympathetic ganglia. Both the ORL1 mRNA splice variants are present in sensory and sympathetic ganglia, however, expression of the shorter ORL1 receptor mRNA dominates over expression of the longer splice variant in rat brain and DRG, whereas, expression of the longer splice variant is dominant in sympathetic ganglia.

摘要

采用逆转录-聚合酶链反应方法，在外周感觉神经节和交感神经节中测定孤啡肽受体1（ORL1）受体mRNA剪接变体的表达，并与三种经典阿片受体亚型（μ、δ和κ）的mRNA表达进行比较。ORL1、μ、δ和κ受体亚型mRNA存在于人类背根神经节（DRG）、三叉神经节以及大鼠DRG中。ORL1、μ和δ受体亚型mRNA存在于大鼠颈上神经节中，而仅ORL1和δ受体mRNA存在于大鼠腰交感神经节中。两种ORL1 mRNA剪接变体均存在于感觉神经节和交感神经节中，然而，较短的ORL1受体mRNA在大鼠脑和DRG中的表达占主导地位，而较长剪接变体的表达在交感神经节中占主导地位。