Mansour A, Fox C A, Burke S, Meng F, Thompson R C, Akil H, Watson S J
Mental Health Research Institute, University of Michigan, Ann Arbor 48109-0720.
J Comp Neurol. 1994 Dec 15;350(3):412-38. doi: 10.1002/cne.903500307.
The mu, delta, and kappa opioid receptors are the three main types of opioid receptors found in the central nervous system (CNS) and periphery. These receptors and the peptides with which they interact are important in a number of physiological functions, including analgesia, respiration, and hormonal regulation. This study examines the expression of mu, delta, and kappa receptor mRNAs in the rat brain and spinal cord using in situ hybridization techniques. Tissue sections were hybridized with 35S-labeled cRNA probes to the rat mu (744-1,064 b), delta (304-1,287 b), and kappa (1,351-2,124 b) receptors. Each mRNA demonstrates a distinct anatomical distribution that corresponds well to known receptor binding distributions. Cells expressing mu receptor mRNA are localized in such regions as the olfactory bulb, caudate-putamen, nucleus accumbens, lateral and medial septum, diagonal band of Broca, bed nucleus of the stria terminalis, most thalamic nuclei, hippocampus, amygdala, medial preoptic area, superior and inferior colliculi, central gray, dorsal and median raphe, raphe magnus, locus coeruleus, parabrachial nucleus, pontine and medullary reticular nuclei, nucleus ambiguus, nucleus of the solitary tract, nucleus gracilis and cuneatus, dorsal motor nucleus of vagus, spinal cord, and dorsal root ganglia. Cellular localization of delta receptor mRNA varied from mu or kappa, with expression in such regions as the olfactory bulb, allo- and neocortex, caudate-putamen, nucleus accumbens, olfactory tubercle, ventromedial hypothalamus, hippocampus, amygdala, red nucleus, pontine nuclei, reticulotegmental nucleus, motor and spinal trigeminal, linear nucleus of the medulla, lateral reticular nucleus, spinal cord, and dorsal root ganglia. Cells expressing kappa receptor mRNA demonstrate a third pattern of expression, with cells localized in regions such as the claustrum, endopiriform nucleus, nucleus accumbens, olfactory tubercle, medial preoptic area, bed nucleus of the stria terminalis, amygdala, most hypothalamic nuclei, median eminence, infundibulum, substantia nigra, ventral tegmental area, raphe nuclei, paratrigeminal and spinal trigeminal, nucleus of the solitary tract, spinal cord, and dorsal root ganglia. These findings are discussed in relation to the physiological functions associated with the opioid receptors.
μ、δ和κ阿片受体是在中枢神经系统（CNS）和外周发现的三种主要阿片受体类型。这些受体及其相互作用的肽在许多生理功能中起重要作用，包括镇痛、呼吸和激素调节。本研究使用原位杂交技术检测大鼠脑和脊髓中μ、δ和κ受体mRNA的表达。组织切片与针对大鼠μ（744 - 1064个碱基）、δ（304 - 1287个碱基）和κ（1351 - 2124个碱基）受体的35S标记的cRNA探针杂交。每种mRNA都表现出独特的解剖分布，与已知的受体结合分布非常吻合。表达μ受体mRNA的细胞定位于嗅球、尾状核 - 壳核、伏隔核、外侧和内侧隔核、布罗卡斜带、终纹床核、大多数丘脑核、海马、杏仁核、内侧视前区、上丘和下丘、中央灰质、背侧和中缝核、中缝大核、蓝斑、臂旁核、脑桥和延髓网状核、疑核、孤束核、薄束核和楔束核、迷走神经背运动核、脊髓和背根神经节等区域。δ受体mRNA的细胞定位与μ或κ不同，表达于嗅球、旧皮质和新皮质、尾状核 - 壳核、伏隔核、嗅结节、腹内侧下丘脑、海马、杏仁核、红核、脑桥核、网状被盖核、运动和脊髓三叉神经核、延髓线性核、外侧网状核、脊髓和背根神经节等区域。表达κ受体mRNA的细胞表现出第三种表达模式，细胞定位于屏状核、内梨状核、伏隔核、嗅结节、内侧视前区、终纹床核、杏仁核、大多数下丘脑核、正中隆起、漏斗、黑质、腹侧被盖区、中缝核、三叉旁核和脊髓三叉神经核、孤束核、脊髓和背根神经节等区域。结合与阿片受体相关的生理功能对这些发现进行了讨论。
