Santis G, Legrand V, Hong S S, Davison E, Kirby I, Imler J L, Finberg R W, Bergelson J M, Mehtali M, Boulanger P
J Gen Virol. 1999 Jun;80 ( Pt 6):1519-1527. doi: 10.1099/0022-1317-80-6-1519.
Adenovirus (Ad) tropism is mediated in part through the fibre protein. The common coxsackie B virus and Ad receptor (CAR) was recently identified as the major receptor for subgroup C Ad serotype 5 (Ad5) and serotype 2 (Ad2) fibres. Effects of mutations in the Ad5 fibre gene were studied to assess domains of the fibre capsomer that could alter virus tropism without altering virus assembly and replication. All mutants that accumulated as fibre monomers failed to assemble with a penton base and proved lethal for Ad5 which suggests that the absence of infectious virions resulted in part from a defect in fibre penton base assembly. Cell binding capacity of all fibre mutants was investigated in cell binding competition experiments with adenovirions using CHO-CAR cells (CHO cells that have been transfected with CAR cDNA and express functional CAR). The results suggest that the R-sheet of the Ad5 fibre knob monomer contains binding motifs for CAR and that beta-strands E and F, or a region close to them, may also be involved in receptor recognition.
腺病毒（Ad）的嗜性部分是通过纤维蛋白介导的。常见的柯萨奇B病毒和Ad受体（CAR）最近被确定为C亚组Ad血清型5（Ad5）和血清型2（Ad2）纤维的主要受体。研究了Ad5纤维基因中的突变效应，以评估纤维衣壳粒中可改变病毒嗜性而不改变病毒组装和复制的结构域。所有以纤维单体形式积累的突变体均无法与五邻体基座组装，对Ad5具有致死性，这表明缺乏感染性病毒粒子部分是由于纤维-五邻体基座组装缺陷所致。在使用CHO-CAR细胞（已转染CAR cDNA并表达功能性CAR的CHO细胞）进行的腺病毒细胞结合竞争实验中，研究了所有纤维突变体的细胞结合能力。结果表明，Ad5纤维球状单体的R片层含有与CAR的结合基序，β链E和F或其附近区域也可能参与受体识别。
