Xu W H
Department of Biology, University of Science and Technology of China, Hefei.
Yi Chuan Xue Bao. 1999;26(2):107-11.
Embryonic diapause in Bombyx is induced by diapause hormone (DH), which is produced in the suboesophageal ganglion from a precursor protein. We have cloned the DH cDNA into pBluescript KS vector that containing T7 RNA promoter can be used to synthesize nonspecific RNA. DH cRNA was synthesized and used as internal control. We quantitatively measured DH mRNA at SG of day 3 pupae by Northern hybridization analysis and suggested that the embryonic diapause of Bombyx in next generation was determined by DH mRNA content in SG.
家蚕的胚胎滞育由滞育激素（DH）诱导产生，该激素由咽下神经节中的一种前体蛋白合成。我们已将DH cDNA克隆到含有T7 RNA启动子的pBluescript KS载体中，该载体可用于合成非特异性RNA。合成了DH cRNA并用作内部对照。通过Northern杂交分析定量测定了3日龄蛹咽下神经节中的DH mRNA，结果表明家蚕下一代的胚胎滞育由咽下神经节中DH mRNA的含量决定。
