Di Cesare P E, Fang C, Leslie M P, Della Valle C J, Gold J M, Tulli H, Perris R, Carlson C S
Musculoskeletal Research Center, Department of Orthopaedic Surgery, New York University Medical Center-Hospital for Joint Diseases, New York 10003, USA.
J Orthop Res. 1999 May;17(3):437-45. doi: 10.1002/jor.1100170321.
Synovium and cartilage from patients with osteoarthritis or rheumatoid arthritis were analyzed for expression of cartilage oligomeric matrix protein. Immunostaining of synovium with antiserum to cartilage oligomeric matrix protein demonstrated positive staining in both diseases. In osteoarthritis, there was positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue. In rheumatoid arthritis, there was less intense staining within the synovial cells and marked intense staining in the deeper connective tissue. In situ hybridization performed with an antisense digoxigenin-labeled riboprobe to human cartilage oligomeric matrix protein confirmed the presence of cartilage oligomeric matrix protein mRNA in the cells of the synovial lining in both types of synovium. Quantitative polymerase chain reaction with a cartilage oligomeric matrix protein MIMIC demonstrated increased cartilage oligomeric matrix protein mRNA in rheumatoid cartilage and synovium as compared with osteoarthritic cartilage and synovium, respectively; mRNA levels in rheumatoid synovium were similar to those from osteoarthritic chondrocytes. As a result of the high expression of cartilage oligomeric matrix protein from rheumatoid synovium, inflammatory synovium should be considered as a potential tissue source of cartilage oligomeric matrix protein in any investigation of biological markers of cartilage metabolism. The upregulated expression of cartilage oligomeric matrix protein in inflammatory tissues suggests its in vivo regulation by cytokines.
对骨关节炎或类风湿性关节炎患者的滑膜和软骨进行分析，以检测软骨寡聚基质蛋白的表达。用抗软骨寡聚基质蛋白抗血清对滑膜进行免疫染色，结果显示在这两种疾病中均呈阳性染色。在骨关节炎中，滑膜细胞及其紧邻的结缔组织呈阳性染色，而深层结缔组织染色较弱。在类风湿性关节炎中，滑膜细胞内染色较弱，而深层结缔组织染色明显较强。用抗地高辛配体标记的人软骨寡聚基质蛋白反义核糖探针进行原位杂交，证实两种滑膜的滑膜衬里细胞中均存在软骨寡聚基质蛋白mRNA。用软骨寡聚基质蛋白MIMIC进行定量聚合酶链反应，结果显示，与骨关节炎软骨和滑膜相比，类风湿性软骨和滑膜中的软骨寡聚基质蛋白mRNA分别增加；类风湿性滑膜中的mRNA水平与骨关节炎软骨细胞中的相似。由于类风湿性滑膜中软骨寡聚基质蛋白的高表达，在任何软骨代谢生物标志物的研究中，炎性滑膜都应被视为软骨寡聚基质蛋白的潜在组织来源。炎性组织中软骨寡聚基质蛋白表达上调表明其在体内受细胞因子调控。
