用于在微秒时间尺度上探测膜动力学的长寿命脂质铼金属-配体配合物。
Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale.
作者信息
Li L, Castellano F N, Gryczynski I, Lakowicz J R
机构信息
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
出版信息
Chem Phys Lipids. 1999 May;99(1):1-9. doi: 10.1016/s0009-3084(99)00002-x.
Abstract
We report the luminescence and spectral properties of a phospholipid analogue containing a long-lifetime luminescent rhenium metal-ligand complex (MLC) covalently linked to the amino group of phosphatidyl ethanolamine. When incorporated into synthetic membranes, this lipid probe displays intensity decay times near 3 microseconds. Importantly, the probe displays highly polarized emission with a maximal fundamental anisotropy of 0.33. This probe is expected to have numerous applications for studies of microsecond diffusion and dynamics of membranes.
摘要
我们报告了一种磷脂类似物的发光和光谱特性，该类似物含有一个与磷脂酰乙醇胺氨基共价连接的长寿命发光铼金属-配体络合物（MLC）。当掺入合成膜中时，这种脂质探针的强度衰减时间接近3微秒。重要的是，该探针显示出高度极化发射，最大基本各向异性为0.33。预计该探针在微秒级膜扩散和动力学研究中有许多应用。
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