在模型膜中使用铼金属-配体脂质共轭物作为供体的共振能量转移研究。
Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane.
作者信息
Li L, Gryczynski I, Lakowicz J R
机构信息
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
出版信息
Chem Phys Lipids. 1999 Sep;101(2):243-53. doi: 10.1016/s0009-3084(99)00066-3.
Abstract
We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 microsecond and lifetime components as long as 3 microseconds in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2 x 10(-8) cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with microsecond lipid probes.
摘要
我们测量了模型膜中供体与受体之间的稳态和时间分辨共振能量转移。供体是一种长寿命的铼 - 脂质复合物，在标记的二油酰磷脂酰胆碱（DOPC）膜中，其平均寿命为1微秒，寿命成分长达3微秒。发现转移效率大大高于不考虑横向扩散时预测的效率。较大的转移效率与膜中相互横向扩散系数接近2×10^(-8) cm²/s一致。这些结果表明，使用微秒级脂质探针可以检测膜中的横向扩散。
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