长寿命脂质金属-配体探针的合成与发光光谱表征
Synthesis and luminescence spectral characterization of long-lifetime lipid metal-ligand probes.
作者信息
Li L, Szmacinski H, Lakowicz J R
机构信息
Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
出版信息
Anal Biochem. 1997 Jan 1;244(1):80-5. doi: 10.1006/abio.1996.9869.
Abstract
We synthesized phospholipid analogues of phosphatidyl ethanolamine which contains a ruthenium metal-ligand complex (MLC) covalently bound to the amino group. Two analogues were synthesized, containing either one (Ru-PE) or two (Ru-PE2) lipid molecules covalently linked to the MLC by the amino group of the lipid. These MLC-lipid probes display intensity decay times from 682 to 357 ns, depending on temperature. Importantly, the luminescence MLC groups display polarized emission, enabling their use for studies of membrane dynamics. The long intensity decay times allowed measurement of the overall rotation correlation time of lipid vesicles to several microseconds. The spectral properties of the model membranes containing Ru-PE or Ru-PE2 were independent of the probe-to-lipid molar ratio from 1:20 to 1:100, suggesting minimal tendency for probe-probe interactions. These MLC-lipid probes can be expected to have numerous applications in studies of membrane dynamics on the microsecond timescale.
摘要
我们合成了磷脂酰乙醇胺的磷脂类似物，其含有与氨基共价结合的钌金属-配体络合物（MLC）。合成了两种类似物，一种含有一个（Ru-PE），另一种含有两个（Ru-PE2）通过脂质氨基与MLC共价连接的脂质分子。这些MLC-脂质探针的强度衰减时间在682至357纳秒之间，具体取决于温度。重要的是，发光MLC基团显示出偏振发射，使其可用于膜动力学研究。长强度衰减时间使得能够测量脂质囊泡的整体旋转相关时间至几微秒。含有Ru-PE或Ru-PE2的模型膜的光谱性质与探针与脂质的摩尔比从1:20至1:100无关，这表明探针-探针相互作用的趋势最小。这些MLC-脂质探针有望在微秒时间尺度的膜动力学研究中有众多应用。
相似文献
1
Synthesis and luminescence spectral characterization of long-lifetime lipid metal-ligand probes.长寿命脂质金属-配体探针的合成与发光光谱表征
Anal Biochem. 1997 Jan 1;244(1):80-5. doi: 10.1006/abio.1996.9869.
2
Long-lifetime metal-ligand pH probe.长寿命金属-配体pH探针。
Anal Biochem. 1997 May 1;247(2):216-22. doi: 10.1006/abio.1997.2057.
3
Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale.用于在微秒时间尺度上探测膜动力学的长寿命脂质铼金属-配体配合物。
Chem Phys Lipids. 1999 May;99(1):1-9. doi: 10.1016/s0009-3084(99)00002-x.
4
A long-lifetime Ru(II) metal-ligand complex as a membrane probe.一种作为膜探针的长寿命钌（II）金属-配体配合物。
Biophys Chem. 1998 Mar 30;71(1):51-62. doi: 10.1016/s0301-4622(97)00135-x.
5
Two dinuclear Ru(II) polypyridyl complexes with different photophysical and cation recognition properties.两种具有不同光物理和阳离子识别特性的双核钌（II）多吡啶配合物。
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 5;136 Pt B:845-51. doi: 10.1016/j.saa.2014.09.103. Epub 2014 Oct 2.
6
High-molecular-weight protein hydrodynamics studied with a long-lifetime metal-ligand complex.利用长寿命金属-配体络合物研究高分子量蛋白质的流体动力学
Biochim Biophys Acta. 2002 Jun 3;1597(2):221-8. doi: 10.1016/s0167-4838(02)00281-9.
7
Fluorescence polarization immunoassay of a high-molecular-weight antigen based on a long-lifetime Ru-ligand complex.基于长寿命钌-配体复合物的高分子量抗原的荧光偏振免疫分析。
Anal Biochem. 1995 May 1;227(1):140-7. doi: 10.1006/abio.1995.1263.
8
Fiber optic lifetime pH sensing based on ruthenium(II) complexes with dicarboxybipyridine.基于钌（II）与二羧基联吡啶配合物的光纤寿命pH传感
Anal Chim Acta. 2008 Sep 19;626(1):62-70. doi: 10.1016/j.aca.2008.07.044. Epub 2008 Aug 6.
9
(trans-1,4-bis[(4-pyridyl)ethenyl]benzene)(2,2'-bipyridine)ruthenium(II) complexes and their supramolecular assemblies with beta-cyclodextrin.（反式-1,4-双[(4-吡啶基)乙烯基]苯）（2,2'-联吡啶）钌（II）配合物及其与β-环糊精的超分子组装体
Inorg Chem. 2004 May 31;43(11):3521-7. doi: 10.1021/ic0352250.
10
Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics.金属-配体配合物作为一类用于蛋白质流体动力学的新型长寿命荧光团。
Biophys J. 1995 Jan;68(1):342-50. doi: 10.1016/S0006-3495(95)80193-1.
引用本文的文献
1
Measurement of the Intensity of Long-Lifetime Luminophores in the Presence of Background Signals Using Phase-Modulation Fluorometry.使用相位调制荧光法在存在背景信号的情况下测量长寿命发光体的强度。
Appl Spectrosc. 1999 Dec;53(12):1490-1495. doi: 10.1366/0003702991946109.
2
Creation of Metal-to-Ligand Charge Transfer Excited States with Two-Photon Excitation.通过双光子激发产生金属-配体电荷转移激发态
Inorg Chem. 1997 Nov 1;36(24):5548-5551. doi: 10.1021/ic970334y. Epub 1997 Nov 19.
3
ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.荧光光谱学进展：多光子激发、工程蛋白、调制传感及微秒级铼金属-配体配合物
Acta Phys Pol A. 1999 Jan;95(1):179-195. doi: 10.12693/APhysPolA.95.179.
4
Tuning photophysical properties with ancillary ligands in Ru(II) mono-diimine complexes.通过钌（II）单二亚胺配合物中的辅助配体调节光物理性质。
J Organomet Chem. 2009 Mar 15;694(6):988-1000. doi: 10.1016/j.jorganchem.2008.11.048. Epub 2008 Nov 30.
5
Membrane Interaction of the Glycosyltransferase WaaG.糖基转移酶WaaG的膜相互作用
Biophys J. 2015 Aug 4;109(3):552-63. doi: 10.1016/j.bpj.2015.06.036.
6
Photophysical studies of bioconjugated ruthenium metal-ligand complexes incorporated in phospholipid membrane bilayers.磷脂膜双层中结合的钌金属配体配合物的光物理研究。
Inorg Chem. 2013 Oct 7;52(19):10835-45. doi: 10.1021/ic400706u. Epub 2013 Sep 24.
7
Dynamics of bacteriophage R17 probed with a long-lifetime Ru(II) metal-ligand complex.用长寿命钌（II）金属配体配合物探测噬菌体 R17 的动力学。
J Fluoresc. 2010 May;20(3):713-8. doi: 10.1007/s10895-010-0612-6. Epub 2010 Feb 27.
8
Binding of hemoglobin to red cell membranes with eosin-5-maleimide-labeled band 3: analysis of centrifugation and fluorescence data.用嗜酸性-5-马来酰亚胺标记的带3血红蛋白与红细胞膜的结合：离心和荧光数据分析
Biochemistry. 2002 Jul 9;41(27):8630-7. doi: 10.1021/bi012007e.
9
High-molecular-weight protein hydrodynamics studied with a long-lifetime metal-ligand complex.利用长寿命金属-配体络合物研究高分子量蛋白质的流体动力学
Biochim Biophys Acta. 2002 Jun 3;1597(2):221-8. doi: 10.1016/s0167-4838(02)00281-9.
10
Lateral diffusion coefficients in membranes measured by resonance energy transfer and a new algorithm for diffusion in two dimensions.通过共振能量转移测量膜中的横向扩散系数以及一种用于二维扩散的新算法。
Biophys J. 2002 Mar;82(3):1358-72. doi: 10.1016/S0006-3495(02)75491-X.
本文引用的文献
1
Influence of Diffusion on Excitation Energy Transfer in Solutions by Gigahertz Harmonic Content Frequency-Domain Fiuorometry.通过千兆赫兹谐波含量频域荧光法研究扩散对溶液中激发能量转移的影响。
J Phys Chem. 1990 Nov 1;94(22):8413-8416. doi: 10.1021/j100385a011.
2
An investigation of squaraines as a new class of fluorophores with long-wavelength excitation and emission.研究方酸作为一类具有长波长激发和发射的新型荧光团。
J Fluoresc. 1993 Sep;3(3):153-5. doi: 10.1007/BF00862734.
3
Review of fluorescence anisotropy decay analysis by frequency-domain fluorescence spectroscopy.频域荧光光谱法荧光各向异性衰减分析的综述。
J Fluoresc. 1993 Jun;3(2):103-16. doi: 10.1007/BF00865324.
4
Synthesis and evaluation of Ru-complexes as anisotropy probes for protein hydrodynamics and immunoassays of high-molecular-weight antigens.钌配合物作为蛋白质流体动力学各向异性探针及高分子量抗原免疫分析的合成与评价
Biophys Chem. 1996 Nov 29;62(1-3):109-20. doi: 10.1016/s0301-4622(96)02199-0.
5
Fluorescence polarization immunoassay of a high-molecular-weight antigen using a long wavelength-absorbing and laser diode-excitable metal-ligand complex.使用长波长吸收且可由激光二极管激发的金属-配体复合物对高分子量抗原进行荧光偏振免疫测定。
Anal Biochem. 1996 Aug 15;240(1):54-9. doi: 10.1006/abio.1996.0330.
6
Percolation and diffusion in three-component lipid bilayers: effect of cholesterol on an equimolar mixture of two phosphatidylcholines.三元脂质双层中的渗透与扩散：胆固醇对两种磷脂酰胆碱等摩尔混合物的影响。
Biophys J. 1993 Feb;64(2):399-412. doi: 10.1016/S0006-3495(93)81381-X.
7
Annexin IV reduces the rate of lateral lipid diffusion and changes the fluid phase structure of the lipid bilayer when it binds to negatively charged membranes in the presence of calcium.膜联蛋白IV在有钙存在的情况下与带负电荷的膜结合时，会降低脂质横向扩散的速率，并改变脂质双层的液相结构。
Biochemistry. 1994 Jul 12;33(27):8225-32. doi: 10.1021/bi00193a008.
8
Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics.金属-配体配合物作为一类用于蛋白质流体动力学的新型长寿命荧光团。
Biophys J. 1995 Jan;68(1):342-50. doi: 10.1016/S0006-3495(95)80193-1.
9
Self-association accompanies inhibition of Ca-ATPase by thapsigargin.毒胡萝卜素抑制钙-ATP酶的同时会发生自我缔合。
Biophys J. 1995 Jan;68(1):208-15. doi: 10.1016/S0006-3495(95)80176-1.
10
Molecular dynamics in mouse atrial tumor sarcoplasmic reticulum.小鼠心房肿瘤肌浆网中的分子动力学
Biophys J. 1995 May;68(5):1787-95. doi: 10.1016/S0006-3495(95)80355-3.
Zotero 插件