Braakhuis B J, Welters M J, Cloos J, Pankras J E, Smeets S J, Fichtinger-Schepman A M
Department of Otolaryngology/Head and Neck Surgery, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.
Cancer Lett. 1999 Apr 26;138(1-2):217-20. doi: 10.1016/s0304-3835(99)00016-6.
The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n = 5) and normal cells (n = 5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition.
本研究的目的是调查正常头颈部上皮细胞（NHNEC）与肿瘤细胞相比，在细胞内铂（Pt）水平和生长抑制方面对顺铂的反应。采用比色法评估暴露于顺铂72小时后的生长抑制情况。肿瘤细胞培养物（n = 5）和正常细胞培养物（n = 5）之间的生长抑制没有差异。用原子吸收光谱法测定的细胞内Pt水平在正常上皮细胞中高出约30倍。本研究的主要发现是，头颈部区域的正常上皮细胞对顺铂的耐受性远高于其肿瘤对应细胞。有趣的是，这一特性对生长抑制没有影响。
