Takeuchi T, Haginaka J
Laboratory of Synthetic Biochemistry, Faculty of Information Sciences, Hiroshima City University, Hiroshima, Japan.
J Chromatogr B Biomed Sci Appl. 1999 May 14;728(1):1-20. doi: 10.1016/s0378-4347(99)00057-2.
Molecular recognition-based separation and sensing systems have received much attention in various fields because of their high selectivity for target molecules. Molecular imprinting has been recognized as a promising technique for the development of such systems, where the molecule to be recognized is added to a reaction mixture of a cross-linker(s), a solvent(s), and a functional monomer(s) that possesses a functional groups(s) capable of interacting with the target molecule. Binding sites in the resultant polymers involve functional groups originating from the added functional monomer(s), which can be constructed according to the shape and chemical properties of the target molecules. After removal of the target molecules, these molecularly imprinted complementary binding sites exhibit high selectivity and affinity for the template molecule. In this article, recent developments in molecularly imprinted polymers are described with their applications as separation media in liquid chromatography, capillary electrophoresis, solid-phase extraction, and membranes. Examples of binding assays and sensing systems using molecularly imprinted polymers are also presented.
基于分子识别的分离和传感系统因其对目标分子具有高选择性而在各个领域备受关注。分子印迹已被公认为是开发此类系统的一种有前途的技术，即将待识别的分子添加到由交联剂、溶剂和具有能够与目标分子相互作用的官能团的功能单体组成的反应混合物中。所得聚合物中的结合位点涉及源自添加的功能单体的官能团，这些官能团可根据目标分子的形状和化学性质构建。去除目标分子后，这些分子印迹的互补结合位点对模板分子表现出高选择性和亲和力。本文介绍了分子印迹聚合物的最新进展及其在液相色谱、毛细管电泳、固相萃取和膜等分离介质中的应用。还列举了使用分子印迹聚合物的结合分析和传感系统的实例。
