Andersson L I
Bioanalytical Chemistry, AstraZeneca R&D Södertälje, Sweden.
J Chromatogr B Biomed Sci Appl. 2000 Aug 4;745(1):3-13. doi: 10.1016/s0378-4347(00)00135-3.
In analytical separation science, molecularly imprinted polymers have been applied in several analytical techniques, such as liquid chromatography, capillary electrochromatography and capillary electrophoresis, solid phase extraction, immunoassay, and as a selective sorbent in chemical sensors. A benefit of imprinted polymers is the possibility to prepare sorbents with selectivity pre-determined for a particular substance, or group of structural analogues. The application most close to a wider acceptance is probably that of solid phase extraction for clean-up of environmental and biological samples. The improved selectivity of imprinted polymers compared with conventional sorbents may lead to cleaner chromatographic traces in the subsequent analytical separation. Furthermore, the solid phase extraction application does not suffer from drawbacks generally associated with imprinted polymers in chromatography, such as peak broadening and tailing. Most liquid chromatographic studies have focused on using imprinted polymers as chiral stationary phases for enantiomer separations. Also, the use of imprinted polymers as selective sorbents in capillary electrochromatography has been presented. For this purpose, a protocol to prepare superporous, monolithic imprinted polymer-based capillary columns has been developed. Due to the high affinities and selectivities often achievable, imprinted polymers have been considered as alternative binding entities in biosensors and in immunoassay type protocols. Here, high stability, easy preparation and ability to be used for assay of both aqueous and organic solvent based samples are advantages of the polymers.
在分析分离科学中，分子印迹聚合物已应用于多种分析技术，如液相色谱、毛细管电色谱和毛细管电泳、固相萃取、免疫测定，以及作为化学传感器中的选择性吸附剂。印迹聚合物的一个优点是有可能制备对特定物质或一组结构类似物具有预先确定选择性的吸附剂。最接近被广泛接受的应用可能是用于环境和生物样品净化的固相萃取。与传统吸附剂相比，印迹聚合物选择性的提高可能会使后续分析分离中的色谱峰更纯净。此外，固相萃取应用不存在通常与色谱中印迹聚合物相关的缺点，如峰展宽和拖尾。大多数液相色谱研究都集中在使用印迹聚合物作为手性固定相进行对映体分离。此外，也有人提出将印迹聚合物用作毛细管电色谱中的选择性吸附剂。为此，已开发出一种制备基于超大孔整体印迹聚合物的毛细管柱的方法。由于通常可实现的高亲和力和选择性，印迹聚合物已被视为生物传感器和免疫测定类型方案中的替代结合实体。在此，聚合物具有高稳定性、易于制备以及能够用于分析水性和有机溶剂基样品的优点。
