Mochizuki T, Kawasaki M, Ishizaki H, Makimura K
Department of Dermatology, Kanazawa Medical University, Ishikawa, Japan.
J Dermatol. 1999 May;26(5):276-81. doi: 10.1111/j.1346-8138.1999.tb03472.x.
Nucleotide sequences of internal transcribed spacer 1 (ITS 1) in nuclear ribosomal DNA from seven morphologically unidentified dermatophyte isolates were determined. The sequences were compared with those of typical isolates of Trichophyton (T.) mentagrophytes var. interdigitale, T. rubrum, and Epidermophyton floccosum. Two of the isolates were classified as T. rubrum and the other five as T. mentagrophytes var. interdigitale. The results did not conflict with identifications using other molecular techniques, including random amplification of polymorphic DNA (RAPD) analysis and restriction enzyme analysis of mitochondrial DNAs. Thus, the nuclotide sequence of ITS 1 is possibly a good molecular marker for identification of these major anthropophilic dermatophyte species.
测定了7株形态学上无法鉴定的皮肤癣菌分离株核糖体DNA内部转录间隔区1（ITS 1）的核苷酸序列。将这些序列与须癣毛癣菌指间变种、红色毛癣菌和絮状表皮癣菌的典型分离株的序列进行了比较。其中2株分离株被分类为红色毛癣菌，另外5株为须癣毛癣菌指间变种。结果与使用其他分子技术（包括随机扩增多态性DNA（RAPD）分析和线粒体DNA限制性酶切分析）的鉴定结果不冲突。因此，ITS 1的核苷酸序列可能是鉴定这些主要亲人性皮肤癣菌物种的良好分子标记。
