从低等白蚁达尔文象白蚁后肠中分离出肠道脱硫弧菌新种。
Isolation of Desulfovibrio intestinalis sp. nov. from the hindgut' of the lower termite Mastotermes darwiniensis.
作者信息
Fröhlich J, Sass H, Babenzien H D, Kuhnigk T, Varma A, Saxena S, Nalepa C, Pfeiffer P, König H
机构信息
Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität, Mainz, Germany.
出版信息
Can J Microbiol. 1999 Feb;45(2):145-52. doi: 10.1139/w98-222.
A Gram-negative, anaerobic sulfate-reducing bacterium was isolated from hindgut contents of the lower termite Mastotermes darwiniensis Froggatt (strain KMS2). Strain KMS2 is motile by a single polar flagellum. The isolate possesses desulfoviridin and catalase activity. The G+C content of its DNA is in the range of 54.5-55.5 mol% (strain KMS2). It respires hydrogen and different low molecular weight organic compounds in the presence of sulfate, thiosulfate, and sulfite, and also oxygen. The isolated strain ferments pyruvate. Fastest growth with a doubling time of 12.5 h was obtained at 37 degrees C and not at 28 degrees C, the temperature at which the termites were grown. The isolate showed a 16S rDNA sequence homology of 95.9% to Desulfovibrio desulfuricans ATCC 27774 and a DNA-DNA homology of 44.6% to D. desulfuricans Essex 6 (type strain). Based on its biochemical properties and 16S rDNA sequence, the isolate was assigned to a new species named Desulfovibrio intestinalis.
从低等白蚁达尔文澳白蚁（Mastotermes darwiniensis Froggatt）（菌株KMS2）的后肠内容物中分离出一种革兰氏阴性、厌氧的硫酸盐还原菌。菌株KMS2通过单根极鞭毛运动。该分离株具有脱硫绿素和过氧化氢酶活性。其DNA的G+C含量在54.5-55.5 mol%范围内（菌株KMS2）。在有硫酸盐、硫代硫酸盐和亚硫酸盐以及氧气存在的情况下，它能利用氢气和不同的低分子量有机化合物进行呼吸。分离出的菌株能发酵丙酮酸。在37℃时获得最快生长，倍增时间为12.5小时，而不是在白蚁生长的28℃。该分离株与脱硫脱硫弧菌（Desulfovibrio desulfuricans）ATCC 27774的16S rDNA序列同源性为95.9%，与脱硫脱硫弧菌埃塞克斯6型（模式菌株）的DNA-DNA同源性为44.6%。基于其生化特性和16S rDNA序列，该分离株被归入一个名为肠道脱硫弧菌（Desulfovibrio intestinalis）的新物种。
Zotero 插件