Takeda T, Takeda S, Saito H, Hamada M, Sawada S
Department of Otolaryngology, Kochi Medical School, Japan.
Acta Otolaryngol. 1999;119(3):341-4. doi: 10.1080/00016489950181369.
Volumetric changes of the scala media were histologically investigated in normal guinea pigs to see whether glycerol-induced volumetric change of the scala media followed a biphasic course similar to the auditory threshold in the glycerol test. Glycerol was administered orally in a 12 ml/kg dose. The volume of the scala media was assessed by examining the cross-sectional area of the scala media in the mid-modiolar sections of the cochlea. Histological study revealed that the time-course of the change in the volume of the scala media after glycerol intake showed biphasic changes. Specifically, the early phase of the glycerol effect is a decrease in endolymph volume. The volume of the scala media significantly decreased by 11.4+/-2.9% 2 h after glycerol intake. Thereafter, the volume began to increase, and reached its peak 6-12 h after intake. In addition, the volume of the scala media significantly increased by 17.6+/-1.1% after 6 h. The present study indicated that the secondary increase in the volume of the scala media following glycerol intake played an important role in the rebound phenomenon in the glycerol test, although the mechanism underlying the hearing loss with the endolymphatic hydrops remains to be elucidated.
为了观察甘油诱导的中阶容积变化是否遵循类似于甘油试验中听阈的双相过程，对正常豚鼠的中阶容积变化进行了组织学研究。以12 ml/kg的剂量口服给予甘油。通过检查耳蜗中轴切片中的中阶横截面积来评估中阶的容积。组织学研究表明，摄入甘油后中阶容积变化的时间过程呈现双相变化。具体而言，甘油效应的早期阶段是内淋巴液体积减少。摄入甘油2小时后，中阶容积显著减少了11.4±2.9%。此后，容积开始增加，并在摄入后6-12小时达到峰值。此外，6小时后中阶容积显著增加了17.6±1.1%。本研究表明，摄入甘油后中阶容积的二次增加在甘油试验的反弹现象中起重要作用，尽管内淋巴积水导致听力损失的潜在机制仍有待阐明。
