D'Errico M, Calcagnile A, Iavarone I, Sera F, Baliva G, Chinni L M, Corona R, Pasquini P, Dogliotti E
Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanitá, Rome, Italy.
Cancer Epidemiol Biomarkers Prev. 1999 Jun;8(6):553-9.
DNA repair capacity (DRC) was studied in 49 patients affected by basal cell carcinoma (BCC) and 68 cancer-free controls belonging to a larger case-control population enrolled for studying BCC risk factors. DRC was measured in the subjects' peripheral blood lymphocytes by using a host-cell reactivation assay that measures cellular activation of a reporter gene irradiated with UV light. A statistically significant age-related decline in DRC was observed in the controls from 20 to 70 years of age but not in the BCC cases. When the DRC values of the BCC patients and controls were compared by age, young BCC cases (age, < or =40 year) repaired less than the controls, although the difference was not statistically significant. Conversely, older BCC patients (age, >40 years) presented an enhanced repair capacity (P < 0.001) as compared with their controls. The search for possible factors associated with the high repair rate of elderly BCC cases revealed that both target cell physiology and life-style habits may affect host DNA repair. Smoking was the variable that explained most of the increase in DRC among older patients. The understanding of how these factors affect host DRC will be relevant for a correct use of this biomarker.
在49例基底细胞癌（BCC）患者和68名无癌对照者中研究了DNA修复能力（DRC），这些患者和对照者来自一个为研究BCC危险因素而招募的更大的病例对照群体。通过使用宿主细胞再激活试验来测量受试者外周血淋巴细胞中的DRC，该试验测量用紫外线照射的报告基因的细胞激活情况。在20至70岁的对照者中观察到DRC存在与年龄相关的统计学显著下降，但在BCC病例中未观察到。当按年龄比较BCC患者和对照者的DRC值时，年轻的BCC病例（年龄≤40岁）的修复能力低于对照者，尽管差异无统计学意义。相反，老年BCC患者（年龄>40岁）与对照者相比，其修复能力增强（P<0.001）。对与老年BCC病例高修复率相关的可能因素的研究表明，靶细胞生理学和生活方式习惯都可能影响宿主DNA修复。吸烟是解释老年患者DRC增加的主要变量。了解这些因素如何影响宿主DRC对于正确使用这种生物标志物具有重要意义。
