柴朴汤（一种东方草药）对束缚水浸应激或乙醇处理诱导的胃损伤的影响。

Yuzurihara M, Ikarashi Y, Kase Y, Torimaru Y, Ishige A, Maruyama Y

Central Research Laboratories, Tsumura and Company, Ibraki, Japan.

J Pharm Pharmacol. 1999 Apr;51(4):483-90. doi: 10.1211/0022357991772547.

The effect of saiboku-to on gastric lesions induced by restraint water-immersion stress and ethanol has been examined in rats. Thirty minutes after oral administration of saiboku-to, the rats were placed in restraint cages and immersed in water at 23 degrees C for 7 h, or orally administered 99.5% ethanol (1 mL) and placed in normal cages for 1 h. The stress for 7 h or the ethanol treatment for 1h induced erosion in the glandular area of the stomach. Histology showed that the surface epithelial cells were desquamated and part of the lamina propria mucosae was injured. The evaluation of lesion index, the cumulative length of the gastric lesion, on the gross appearance of the stomach, revealed that saiboku-to dose-dependently inhibited both the water-immersion stress-induced gastric erosion and ethanol-induced gastric erosion. To determine whether the anti-erosion effect of saiboku-to was because of a mild irritant effect, saiboku-to or 20% ethanol, which is known as a typical mild irritant, were given orally. After 30 min a strong irritant, 99.5% ethanol, was given orally. Histological examination was performed 30 min after administration of saiboku-to or the mild irritant, and 1 h after administration of the strong irritant. The mild irritant induced a reduction in surface epithelial cells 30 min after administration. Furthermore, the mild irritant protected the stomach against mucosal erosion produced by the strong irritant. Saiboku-to protected the strong irritant-induced erosion without producing mild irritation as observed in stomach treated with 20% ethanol. Pretreatment with saiboku-to also inhibited the decrease in the levels of hexosamine, gastric mucus glycoprotein, induced by the strong irritant. In pylorus-ligated rats, saiboku-to dose-dependently inhibited gastric acid secretion, a gastric aggressive factor. These results suggest that the anti-erosion effect of saiboku-to which is not a mild irritant, involves both inhibition of aggressive factors, such as gastric acid secretion, and augmentation of defensive factors, such as gastric mucus cells.

在大鼠中研究了柴朴汤对束缚水浸应激和乙醇诱导的胃损伤的影响。口服柴朴汤30分钟后，将大鼠置于束缚笼中，于23℃水中浸泡7小时，或口服99.5%乙醇（1 mL）并置于正常笼中1小时。7小时的应激或1小时的乙醇处理均导致胃腺区出现糜烂。组织学检查显示，表面上皮细胞脱落，固有层黏膜部分受损。通过评估胃损伤指数（即胃损伤的累积长度），发现柴朴汤剂量依赖性地抑制了水浸应激诱导的胃糜烂和乙醇诱导的胃糜烂。为确定柴朴汤的抗糜烂作用是否源于轻度刺激作用，口服给予柴朴汤或已知为典型轻度刺激剂的20%乙醇。30分钟后口服强刺激剂99.5%乙醇。在给予柴朴汤或轻度刺激剂30分钟后以及给予强刺激剂1小时后进行组织学检查。轻度刺激剂给药30分钟后导致表面上皮细胞减少。此外，轻度刺激剂可保护胃免受强刺激剂引起的黏膜糜烂。柴朴汤可保护胃免受强刺激剂诱导的糜烂，且不会像用20%乙醇处理的胃那样产生轻度刺激。柴朴汤预处理还可抑制强刺激剂诱导的己糖胺、胃黏液糖蛋白水平的降低。在幽门结扎的大鼠中，柴朴汤剂量依赖性地抑制胃酸分泌（一种胃攻击因子）。这些结果表明，柴朴汤的抗糜烂作用并非源于轻度刺激，而是涉及对胃酸分泌等攻击因子的抑制以及胃黏液细胞等防御因子的增强。