蔷薇科果树中多酚氧化酶的免疫学和分子比较

Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees.

作者信息

Haruta M, Murata M, Kadokura H, Homma S

机构信息

Department of Nutrition and Food Science, Ochanomizu University, Tokyo, Japan.

出版信息

Phytochemistry. 1999 Mar;50(6):1021-5. doi: 10.1016/s0031-9422(98)00633-5.

DOI:10.1016/s0031-9422(98)00633-5

PMID:10385997

Abstract

An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.

摘要

一种针对苹果多酚氧化酶（PPO）产生的抗体与来自日本梨（Pyrus pyrifolia）、西洋梨（Pyrus communis）、桃（Prunus persica）、中国榅桲（Pseudocydonia sinensis）和日本枇杷（Eriobotrya japonica）的PPO发生交叉反应。扩增并鉴定了相应PPO基因的核心片段（681 bp）。推导的蛋白质序列显示同一性为85.3%至97.5%。当在pH 4下测定时，这些PPO的绿原酸氧化酶活性比在pH 6下测定时更高。这些结果表明蔷薇科植物中的PPO在结构和酶活性方面相似。