Böttcher P, Maierl J
Institute of Veterinary Anatomy, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, München, Germany.
Anat Histol Embryol. 1999 May;28(2):97-102. doi: 10.1046/j.1439-0264.1999.00171.x.
Using a new method derived from the 'visible human project' (Spitzer et al., 1996, Journal of the American Medical Informatics Association, 3, 118-130), we were able to establish a simple and low-cost tool which produces high-quality cryosections of macroscopic specimens down to 1-mm slice thickness, based on a milling process. For the first time, a macroscopic cryotome is available to veterinary anatomists, which can be used on cutting faces up to 25 cm high and 50 cm wide and with a minimal slice thickness of 1 mm without any gap. The method employs a modified wood circular saw. Recording of the cutting faces is carried out 'online' by a high-resolution digital camera. The process has been tested extensively and produces high-quality sections of very hard material (teeth) as well as of very soft tissues (brain). It is now possible in veterinary medicine to provide three-dimensional anatomical databases of high resolution and of tissue-specific colour as an additional tool for high-quality two- and three-dimensional anatomical reconstructions for use in science and education.
利用一种源自“可视人计划”（斯皮策等人，1996年，《美国医学信息学协会杂志》，第3卷，第118 - 130页）的新方法，我们得以建立一种简单且低成本的工具，该工具基于铣削工艺，能够制作出厚度低至1毫米的宏观标本高质量冷冻切片。首次为兽医解剖学家提供了一种宏观冷冻切片机，它可用于切割高达25厘米高、50厘米宽的表面，且切片厚度最小为1毫米，无任何间隙。该方法采用了改良的木工圆锯。切割面的记录由高分辨率数码相机“在线”进行。该过程已得到广泛测试，能制作出非常坚硬的材料（牙齿）以及非常柔软的组织（大脑）的高质量切片。现在兽医学中有可能提供高分辨率且具有组织特异性颜色的三维解剖数据库，作为用于科学和教育的高质量二维和三维解剖重建的额外工具。
