一项关于裂解酶对大鼠肝脏和肾脏质膜酶活性影响的组织化学研究。

A histochemical study about the influence of lytic enzymes on plasma membrane enzyme activities in rat liver and kidney.

Hardonk M J, Meskendorp-Haarsma T J, Koudstaal J

Histochemistry. 1978 Dec 1;58(3):177-81. doi: 10.1007/BF00495717.

Abstract

The effect of lipolytic, glycolytic and proteolytic enzymes on the activities of plasma membrane enzyme activities in rat liver and kidney has been investigated by a pretreatment of tissue sections with the lytic enzymes. 2. The action of the proteolytic enzymes causes a very strong decrease of leucyl-beta-naphthylamidase activity, whereas the activities of ATP-ase, 5'-nucleotidase and alkaline phosphatase show a lesser decrease. This indicates a different membrane anchorage of leucyl-beta-naphthylamidase as compared to that of the phosphatases. 3. Treatment with glycolytic enzymes results in a decrease of 5'-nucleotidase and ATP-ase activity, whereas liver alkaline phosphatase and leucyl-beta-naphthylamidase show an increase in activity. 4. Treatment with phospholipase C gives about the same results. The very strong decrease of 5'-nucleotidase activity indicates a great dependence on phospholipids.

摘要

通过用裂解酶对组织切片进行预处理，研究了脂解酶、糖酵解酶和蛋白水解酶对大鼠肝脏和肾脏质膜酶活性的影响。2. 蛋白水解酶的作用导致亮氨酰-β-萘胺酶活性大幅下降，而ATP酶、5'-核苷酸酶和碱性磷酸酶的活性下降幅度较小。这表明亮氨酰-β-萘胺酶与磷酸酶的膜锚定方式不同。3. 用糖酵解酶处理导致5'-核苷酸酶和ATP酶活性下降，而肝脏碱性磷酸酶和亮氨酰-β-萘胺酶活性增加。4. 用磷脂酶C处理得到大致相同的结果。5'-核苷酸酶活性的大幅下降表明其对磷脂有很大依赖性。