Kato S, Aoyama K, Nakamura T, Ichihara A
J Biochem. 1979 Nov;86(5):1419-25. doi: 10.1093/oxfordjournals.jbchem.a132659.
When liver cells were dispersed with collagenase, their 5'-nucleotidase activity decreased to half the initial level, but it increased to the original level again on culture of the cells for a few days. The activity of another membrane enzyme, alkaline phosphatase, did not decrease on dispersion of the cells, but it increased about 10-fold on culture of the cells. These inductions did not require any hormone, but the effects were greater at a high cell density. These enzymes are located in both the plasma membranes and the cytoplasm, but the enzymes in these two locations can be distinguished by differences in their pH optima, substrate specificities, and susceptibilities to inhibitors. The increases were found to be due to increases in the activity of only the enzymes in the plasma membranes. The increases in enzyme activities were inhibited by actinomycin D, cycloheximide, and puromycin. The activities of leucine aminopeptidase and aminopeptidase B, other membrane enzymes, remained constant during dispersion and culture of the cells. These results show that enzymes in the cell membranes are affected in different ways by cell dispersion with collagenase and subsequent culture of the cells.
当用胶原酶分散肝细胞时，其5'-核苷酸酶活性降至初始水平的一半，但在细胞培养几天后又恢复到原来的水平。另一种膜酶碱性磷酸酶的活性在细胞分散时并未降低，但在细胞培养时增加了约10倍。这些诱导不需要任何激素，但在高细胞密度下效果更明显。这些酶存在于质膜和细胞质中，但这两个位置的酶可以通过它们的最适pH值、底物特异性和对抑制剂的敏感性差异来区分。发现增加仅仅是由于质膜中酶的活性增加。酶活性的增加受到放线菌素D、环己酰亚胺和嘌呤霉素的抑制。其他膜酶亮氨酸氨肽酶和氨肽酶B的活性在细胞分散和培养过程中保持不变。这些结果表明，细胞膜中的酶在通过胶原酶进行细胞分散以及随后的细胞培养过程中受到不同方式的影响。
