Kumpulainen T, Korhonen L K
Histochemistry. 1978 Dec 1;58(3):183-92. doi: 10.1007/BF00495718.
Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Flurorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.
采用阿姆斯特朗等人（1966年）以及伯恩斯坦和施雷尔（1972年）改进方法纯化的人红细胞碳酸酐酶B（HCA B）对兔子进行免疫。通过硫酸铵沉淀分离球蛋白部分。当使用欧克托洛尼双扩散技术和免疫电泳进行判断时，抗HCA B球蛋白具有特异性。未发现与人红细胞碳酸酐酶C的交叉反应，但观察到与大鼠、小鼠和豚鼠的红细胞碳酸酐酶存在交叉反应。异硫氰酸荧光素偶联的山羊抗兔球蛋白用于在组织切片和载玻片上的红细胞中定位HCA B。
