Phillipson J D, Handa S S, Gorrod J W
J Pharm Pharmacol. 1976 Sep;28(9):687-91. doi: 10.1111/j.2042-7158.1976.tb02837.x.
Incubation of guinea-pig liver microsomal preparations with atropine or hyoscine resulted in the formation of the corresponding nor-alkaloids and both isomers of atropine N-oxide from atropine and one isomer of hyoscine N-oxide from hyoscine. Separate incubations of guinea-pig liver microsomal preparations with nor-atropine and nor-hyoscine yielded the corresponding hydroxylamines. The N-oxide and hydroxylamine metabolites were identified by comparison of their t.l.c. behavior and m.x. with prepared compounds and also by their reduction to the corresponding tertiary or secondary amines.
将豚鼠肝微粒体制剂与阿托品或东莨菪碱一起温育，会导致相应去甲生物碱的形成，阿托品会生成阿托品N-氧化物的两种异构体，东莨菪碱会生成东莨菪碱N-氧化物的一种异构体。将豚鼠肝微粒体制剂分别与去甲阿托品和去甲东莨菪碱温育，会产生相应的羟胺。通过比较它们的薄层层析行为和熔点与制备的化合物，并通过将它们还原为相应的叔胺或仲胺，来鉴定N-氧化物和羟胺代谢物。
