Limawongpranee S, Hayashidani H, Okatani A T, Hirota C, Kaneko K, Ogawa M
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Avian Dis. 1999 Apr-Jun;43(2):302-9.
Cecal and environmental samples were collected and examined for the presence of Salmonella on a farm where a high prevalence of Salmonella blockley in chickens was observed. Of 895 cecal and 525 environmental samples examined, 242 (27.0%) and 202 (38.5%) samples, respectively, yielded S. blockley. From the analysis of plasmid profile patterns, 11 different plasmid profile patterns were found among 444 isolates, with plasmid patterns c and d the most frequent among the isolates from ceca and the environment. Salmonella blockley was isolated from environmental samples such as floor litter, walls, drinking water, waste water, dust, and soil collected when barns were occupied and was positive in drinking water, waste water, and soil when samples were collected from empty barns with occupied neighboring barns, but it was negative in all environmental samples with the exception of soil when the environmental samples were collected from empty barns with empty neighboring barns.
在一个观察到鸡群中布氏沙门氏菌高流行率的农场，采集了盲肠和环境样本并检测其中是否存在沙门氏菌。在检测的895份盲肠样本和525份环境样本中，分别有242份（27.0%）和202份（38.5%）样本检出布氏沙门氏菌。通过质粒图谱模式分析，在444株分离株中发现了11种不同的质粒图谱模式，其中质粒模式c和d在盲肠和环境分离株中最为常见。布氏沙门氏菌从占用鸡舍时收集的地面垫料、墙壁、饮用水、废水、灰尘和土壤等环境样本中分离得到，当从相邻鸡舍被占用的空鸡舍采集样本时，饮用水、废水和土壤检测呈阳性，但当从相邻鸡舍为空的空鸡舍采集环境样本时，除土壤外所有环境样本检测均为阴性。
