Vollmer C M, Eilber F C, Butterfield L H, Ribas A, Dissette V B, Koh A, Montejo L D, Lee M C, Andrews K J, McBride W H, Glaspy J A, Economou J S
Division of Surgical Oncology, University of California Los Angeles, 90095-1782, USA.
Cancer Res. 1999 Jul 1;59(13):3064-7.
The majority of human hepatocellular carcinomas overexpress alpha-fetoprotein (AFP). Two genetic immunization strategies were used to determine whether AFP could serve as a target for T-cell immune responses. Dendritic cells engineered to express AFP produced potent T-cell responses in mice, as evidenced by the generation of AFP-specific CTLs, cytokine-producing T cells, and protective immunity. AFP plasmid-based immunization generated less potent responses. These novel observations demonstrate that this oncofetal antigen can serve as an effective tumor rejection antigen. This provides a rational, gene therapy-based strategy for this disease, which is responsible for the largest number of cancer-related deaths worldwide.
大多数人类肝细胞癌会过度表达甲胎蛋白（AFP）。采用了两种基因免疫策略来确定AFP是否可作为T细胞免疫反应的靶点。经基因工程改造以表达AFP的树突状细胞在小鼠体内产生了强烈的T细胞反应，这可通过产生AFP特异性CTL、产生细胞因子的T细胞以及保护性免疫来证明。基于AFP质粒的免疫产生的反应较弱。这些新发现表明，这种癌胚抗原有望成为一种有效的肿瘤排斥抗原。这为这种在全球导致癌症相关死亡人数最多的疾病提供了一种合理的、基于基因治疗的策略。
