Samuelsen A B, Cohen E H, Paulsen B S, Brüll L P, Thomas-Oates J E
School of Pharmacy, Department of Pharmacognosy, University of Oslo, Norway.
Carbohydr Res. 1999 Feb 28;315(3-4):312-8. doi: 10.1016/s0008-6215(99)00038-5.
The seed mucilage from Plantago major L. contains acidic heteroxylan polysaccharides. For further structural analysis, oligosaccharides were generated by partial acid hydrolysis and then isolated by high-pH anion-exchange chromatography (HPAEC). Each HPAEC fraction was shown by ESMS to contain one major oligosaccharide and several minor components. Partial structures of the oligosaccharides were determined using GC-MS, ESMS and ES tandem mass spectrometry (ESMS/MS). A (1-->4)-linked xylan trisaccharide and (1-->3)-linked xylan oligosaccharides with DP 6-11 suggested that the backbone of the heteroxylan polysaccharide consisted of blocks of (1-->4)-linked and (1-->3)-linked Xylp residues. A (1-->2)-linked Xylp disaccharide and a branched tetrasaccharide were also found, revealing that single Xylp residues are linked to the O-2 of some of the (1-->4)-linked Xylp residues in the backbone. In addition, our results confirm the presence of side chains consisting of the disaccharide GlcpA-(1-->3)-Araf.
大车前（Plantago major L.）的种子黏液含有酸性杂木聚糖多糖。为了进行进一步的结构分析，通过部分酸水解生成寡糖，然后通过高pH阴离子交换色谱法（HPAEC）进行分离。电喷雾质谱（ESMS）显示，每个HPAEC级分均含有一种主要寡糖和几种次要成分。使用气相色谱 - 质谱联用仪（GC-MS）、电喷雾质谱（ESMS）和电喷雾串联质谱（ESMS/MS）确定了寡糖的部分结构。一个（1→4）连接的木聚糖三糖和聚合度为6 - 11的（1→3）连接的木聚糖寡糖表明，杂木聚糖多糖的主链由（1→4）连接和（1→3）连接的木糖残基块组成。还发现了一个（1→2）连接的木糖二糖和一个分支四糖，这表明单个木糖残基与主链中一些（1→4）连接的木糖残基的O-2相连。此外，我们的结果证实了由二糖葡萄糖醛酸 - （1→3） - 阿拉伯糖组成的侧链的存在。
