Gomes T, Caponio F
Istituto di Produzioni e Preparazioni Alimentari, Università di Bari, Foggia, Italy.
J Chromatogr A. 1999 Jun 4;844(1-2):77-86. doi: 10.1016/s0021-9673(99)00321-0.
This paper proposes an analytical method to evaluate the classes of products of polymerization, oxidation and hydrolysis as well as the polar compounds present in refined edible oils in a more reliable fashion. The polar compounds of a marketed refined peanut oil were analyzed by preparative gel permeation chromatography and the classes of substances corresponding to single chromatogram peaks were collected by means of a fraction collector, purified and used as standards for high-performance size-exclusion chromatographic analysis. The linearity of detector response, the precision and accuracy of the method for each class of compounds and for polar compounds were assessed. Another aim was to verify whether this method may be applied to other refined peanut oils and to edible vegetable oils in general, even of different botanical origin, using the standards that had already been prepared for that particular peanut oil. The results obtained showed that this was possible and the analytical method developed can be extended to the most common edible vegetable oils.
本文提出了一种分析方法，以更可靠的方式评估聚合、氧化和水解产物的类别以及精炼食用油中存在的极性化合物。通过制备型凝胶渗透色谱法分析市售精炼花生油中的极性化合物，并借助馏分收集器收集与单个色谱峰相对应的物质类别，进行纯化后用作高效尺寸排阻色谱分析的标准品。评估了检测器响应的线性、该方法对各类化合物以及极性化合物的精密度和准确度。另一个目的是验证该方法是否可以应用于其他精炼花生油以及一般的食用植物油，即使它们来自不同的植物来源，使用已经为该特定花生油制备的标准品。获得的结果表明这是可行的，并且所开发的分析方法可以扩展到最常见的食用植物油。
