Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Campus Universidad Pablo de Olavide, Ctra. de Utrera, km 1, E-41013, Sevilla, Spain.
Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Campus Universidad Pablo de Olavide, Ctra. de Utrera, km 1, E-41013, Sevilla, Spain.
J Chromatogr A. 2018 Apr 27;1547:62-70. doi: 10.1016/j.chroma.2018.03.014. Epub 2018 Mar 11.
The objective of the present study was to explore the possibilities of the direct analysis of vegetable oils by normal-phase HPLC-DAD to evaluate the amounts of the main oxidation products of triacylglycerols containing linoleate, i.e. hydroperoxy-, keto- and hydroxy-dienes. A follow-up of oxidation at 40 °C of trilinolein, used as a simplified model lipid system, high-linoleic sunflower oil and high-oleic sunflower oil was performed to evaluate samples with different fatty acid compositions and different oxidation levels. The results showed that the HPLC-DAD method proposed allows for determining the concentrations of mono-hydroperoxydienes in edible oils without applying any isolation or derivatization step. The method was found to be direct, sensitive (LOQ 0.06 mmol/kg oil), precise (CV ≤ 5%) and also accurate, with 99% of analyte recovery. It also enabled the estimation of the minor amounts of ketodienes, but not those of hydroxydienes, which presented wide chromatographic bands and coeluted with a number of different minor oxidation compounds.
本研究旨在探索正相高效液相色谱-二极管阵列检测（HPLC-DAD）直接分析植物油的可能性，以评估含有亚油酸的三酰基甘油主要氧化产物（即氢过氧化物、酮和羟基二烯）的含量。本研究还对三亚油酸甘油酯（用作简化的模型脂质体系）、高亚油酸葵花籽油和高油酸葵花籽油在 40°C 下的氧化情况进行了后续跟踪，以评估具有不同脂肪酸组成和不同氧化水平的样品。结果表明，所提出的 HPLC-DAD 方法可在不进行任何分离或衍生化步骤的情况下，测定食用油中单氢过氧化物二烯的浓度。该方法具有直接、灵敏（LOQ 为 0.06mmol/kg 油）、精确（CV≤5%）和准确的特点，分析物回收率为 99%。该方法还可以估计少量的酮二烯，但不能估计羟基二烯的含量，因为羟基二烯的色谱带较宽，与许多不同的少量氧化化合物共洗脱。
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