离子交换色谱分离生物胺的冷凝成核光散射检测

Condensation nucleation light scattering detection for biogenic amines separated by ion-exchange chromatography.

作者信息

Sadain S K, Koropchak J A

机构信息

Department of Chemistry and Biochemistry, Southern Illinois University at Carbondale 62901-4409, USA.

出版信息

J Chromatogr A. 1999 Jun 4;844(1-2):111-8. doi: 10.1016/s0021-9673(99)00387-8.

DOI:10.1016/s0021-9673(99)00387-8

PMID:10399327

Abstract

A sensitive method of analysis for biogenic amines, putrescine, cadaverine, histamine and an amino acid precursor, histidine is described herein using ion-exchange chromatography and condensation nucleation light scattering detection. The method was successfully used for the analysis of biogenic amines in fish samples. The method offers a number of advantages: fast elution of analytes with no need for mobile phase conductivity suppression, no derivatization and no electrochemical activity for the analyte's detection. The 3 sigma detection limits for these compounds were found to range from 8 to 20 ng/ml.

摘要

本文介绍了一种用于分析生物胺、腐胺、尸胺、组胺以及一种氨基酸前体——组氨酸的灵敏方法，该方法采用离子交换色谱和凝聚核光散射检测。此方法已成功用于鱼类样品中生物胺的分析。该方法具有诸多优点：分析物洗脱速度快，无需抑制流动相的电导率，无需衍生化，且分析物检测无需电化学活性。这些化合物的3σ检测限为8至20 ng/ml。