Malle P, Vallé M, Bouquelet S
Centre National d'Etudes Vétérinaires et Alimentaires, Boulogne sur Mer, France.
J AOAC Int. 1996 Jan-Feb;79(1):43-9.
A liquid chromatographic (LC) method is described for quantitative determination of putrefaction amines: putrescine, cadaverine, histamine, spermidine, and spermine. These amines are extracted from fish by grinding with perchloric acid at -20 degrees C. The amines are reacted with dansyl chloride at alkaline pH, dried under a stream of nitrogen, placed in an automatic injector at -20 degrees C, and separated in a Kromasil C18 reversed-phase column at 25 degrees C. A new gradient elutes the amines in 22 min and regenerates the column in 30 min. Regression lines are obtained with high coefficients of correlation. This method is faster and more reproducible than other methods and shows that the quality index is a reliable, albeit species-specific, criterion of fish decomposition.
本文描述了一种用于定量测定腐败胺类物质（腐胺、尸胺、组胺、亚精胺和精胺）的液相色谱（LC）方法。这些胺类物质通过在-20℃下用高氯酸研磨从鱼中提取。胺类物质在碱性pH下与丹磺酰氯反应，在氮气流下干燥，在-20℃下置于自动进样器中，并在25℃下于Kromasil C18反相柱中分离。一种新的梯度在22分钟内洗脱胺类物质，并在30分钟内使柱子再生。获得了具有高相关系数的回归线。该方法比其他方法更快且更具重现性，表明质量指标是鱼类腐败的一个可靠但因物种而异的标准。
