Zhao Xinying, Jiao Xia, Xia Min, Liu Qing, Zhang Jinghua
Beijing Center for Physical and Chemical Analysis, Beijing, China.
Se Pu. 2009 Jul;27(4):505-8.
An ion chromatographic (IC) method was developed for the separation of five biogenic amines (BAs), including putrescine, cadaverine, histamine, spermidine and spermine, in source water. The sample was separated on a TSK-GEL SuperIC cation exchange column (150 mm x 4.6 mm) after filtering treatment by 0.45 microm inorganic membrane. Methanesulfonic acid was used as eluent for concentration gradient elution at the rate of 1.0 mL/min. The injection volume was 100 microL and the BAs were detected by a non-suppressed conductivity detector. The result showed that the baseline separation was obtained for the 5 kinds of BAs. The linear relationships between the concentration and the peak area were obtained in the concentration range of 1.0 - 30.0 mg/L. The relative standard deviations (RSDs) of retention time and peak area were no more than 0.02% and 2.08%, respectively. The recoveries were from 96.0% to 107.0%. The method is simple, rapid, highly sensitive and with good repeatability. It could be used for the determination the BAs in the source water.
建立了一种离子色谱（IC）法，用于分离源水中的五种生物胺（BA），包括腐胺、尸胺、组胺、亚精胺和精胺。样品经0.45微米无机膜过滤处理后，在TSK-GEL SuperIC阳离子交换柱（150 mm×4.6 mm）上进行分离。以甲磺酸为洗脱剂，采用浓度梯度洗脱，流速为1.0 mL/min。进样量为100微升，采用非抑制型电导检测器检测生物胺。结果表明，5种生物胺实现了基线分离。在1.0 - 30.0 mg/L浓度范围内，浓度与峰面积呈线性关系。保留时间和峰面积的相对标准偏差（RSD）分别不超过0.02%和2.08%。回收率为96.0%至107.0%。该方法简单、快速、灵敏度高且重复性好。可用于源水中生物胺的测定。
