Torres M, Goldberg J, Jensen T E
Department of Biological Sciences, Lehman College, City University of New York, Bronx, USA.
Microbios. 1998;96(385):141-7.
The study was conducted to determine whether living or killed cell polyphosphate bodies (PPB) would sequester more of several heavy metals. Living and heat- or glutaraldehyde-killed cells were exposed to 20 ppm of Zn, Pb, Mn and Al. Air-dried cells on Formvar-coated grids were first observed in the transmission electron microscope. The unit was then switched to the scanning transmission mode of operation with the spot setting. X-rays were collected on an energy dispersive X-ray spectrometer and mass fractions of the metals were determined. In all cases live cells sequestered a larger amount of the metal than cells killed by boiling or with glutaraldehyde. In all cases the cells killed by glutaraldehyde sequestered more of the heavy metals than cells killed by boiling. The results of the investigation show that PPB in living cells with active uptake systems take up and sequester more of the metals Zn, Pb, Mn and Al than killed cells.
进行该研究是为了确定活细胞或灭活细胞的多聚磷酸盐体（PPB）是否会螯合更多的几种重金属。将活细胞以及经加热或戊二醛处理灭活的细胞暴露于20 ppm的锌、铅、锰和铝中。首先在透射电子显微镜下观察涂有福尔瓦（Formvar）膜的网格上的空气干燥细胞。然后将仪器切换到扫描透射操作模式并设置光斑。在能量色散X射线光谱仪上收集X射线，并测定金属的质量分数。在所有情况下，活细胞螯合的金属量都比煮沸或用戊二醛处理灭活的细胞多。在所有情况下，经戊二醛处理灭活的细胞螯合的重金属都比煮沸处理灭活的细胞多。调查结果表明，具有活跃摄取系统的活细胞中的PPB比灭活细胞摄取和螯合更多的锌、铅、锰和铝等金属。
