使用扫描透射电子显微镜能谱仪（STEM EDX）对细菌多聚磷酸盐体的元素组成和质量进行定量分析。
Quantitative analysis of the elemental composition and the mass of bacterial polyphosphate bodies using STEM EDX.
作者信息
Goldberg J, Gonzalez H, Jensen T E, Corpe W A
机构信息
Department of Biological Sciences, Lehman College, Bronx, New York 10468, USA.
出版信息
Microbios. 2001;106(415):177-88.
The quantitative analysis of laboratory grown organisms (Plectonema boryanum and Staphylococcus aureus) revealed that a typical in vivo polyphosphate body (PPB) contains O (4.3 x 10(-8) microg), C (1.2 x 10(-8) microg), P (6.7 x 10(-9) microg), Mg (1.3 x 10(-9) microg), Ca (6.7 x 10(-10) microg), K (6.7 x 10(-10) microg), Fe (6.0 x 10(-10) microg), S (5.4 x 10(-10) microg) and Al (5.9 x 10(-10) microg). Quantitative X-ray analysis of samples from nature showed PPB contain O (1.63 x 10(-8) microg), C (4.75 x 10(-9) microg), P (2.50 x 10(-9) microg), Mg (5.0 x 10(-10) microg), Ca (2.50 x 10(-10) microg), K (2.50 x 10(-10) microg), Fe (2.25 x 10(-10) microg) and S (2.0 x 10(-10) microg). The mass of an average polyphosphate body was 6.7 x 10(-8) microg for P. boryanum, 2.5 x 10(-8) microg for S. aureus and for microbes from the natural environment 6.3 x 10(-8) microg. The results indicate that the PPB may have other unknown functions in addition to essential element storage, acting as a detoxification method by sequestering heavy metals and providing a homeostasis system in the cell.
对实验室培养的生物体（颤藻和金黄色葡萄球菌）进行的定量分析表明，一个典型的体内多聚磷酸盐体（PPB）含有氧（4.3×10⁻⁸微克）、碳（1.2×10⁻⁸微克）、磷（6.7×10⁻⁹微克）、镁（1.3×10⁻⁹微克）、钙（6.7×10⁻¹⁰微克）、钾（6.7×10⁻¹⁰微克）、铁（6.0×10⁻¹⁰微克）、硫（5.4×10⁻¹⁰微克）和铝（5.9×10⁻¹⁰微克）。对天然样品的定量X射线分析表明，PPB含有氧（1.63×10⁻⁸微克）、碳（4.75×10⁻⁹微克）、磷（2.50×10⁻⁹微克）、镁（5.0×10⁻¹⁰微克）、钙（2.50×10⁻¹⁰微克）、钾（2.50×10⁻¹⁰微克）、铁（2.25×10⁻¹⁰微克）和硫（2.0×10⁻¹⁰微克）。平均一个多聚磷酸盐体的质量，颤藻为6.7×10⁻⁸微克，金黄色葡萄球菌为2.5×10⁻⁸微克，天然环境中的微生物为6.3×10⁻⁸微克。结果表明，除了储存必需元素外，PPB可能还有其他未知功能，它可通过螯合重金属作为一种解毒方法，并在细胞中提供一个稳态系统。
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