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使用扫描透射电子显微镜能谱仪(STEM EDX)对细菌多聚磷酸盐体的元素组成和质量进行定量分析。

Quantitative analysis of the elemental composition and the mass of bacterial polyphosphate bodies using STEM EDX.

作者信息

Goldberg J, Gonzalez H, Jensen T E, Corpe W A

机构信息

Department of Biological Sciences, Lehman College, Bronx, New York 10468, USA.

出版信息

Microbios. 2001;106(415):177-88.

PMID:11522129
Abstract

The quantitative analysis of laboratory grown organisms (Plectonema boryanum and Staphylococcus aureus) revealed that a typical in vivo polyphosphate body (PPB) contains O (4.3 x 10(-8) microg), C (1.2 x 10(-8) microg), P (6.7 x 10(-9) microg), Mg (1.3 x 10(-9) microg), Ca (6.7 x 10(-10) microg), K (6.7 x 10(-10) microg), Fe (6.0 x 10(-10) microg), S (5.4 x 10(-10) microg) and Al (5.9 x 10(-10) microg). Quantitative X-ray analysis of samples from nature showed PPB contain O (1.63 x 10(-8) microg), C (4.75 x 10(-9) microg), P (2.50 x 10(-9) microg), Mg (5.0 x 10(-10) microg), Ca (2.50 x 10(-10) microg), K (2.50 x 10(-10) microg), Fe (2.25 x 10(-10) microg) and S (2.0 x 10(-10) microg). The mass of an average polyphosphate body was 6.7 x 10(-8) microg for P. boryanum, 2.5 x 10(-8) microg for S. aureus and for microbes from the natural environment 6.3 x 10(-8) microg. The results indicate that the PPB may have other unknown functions in addition to essential element storage, acting as a detoxification method by sequestering heavy metals and providing a homeostasis system in the cell.

摘要

对实验室培养的生物体(颤藻和金黄色葡萄球菌)进行的定量分析表明,一个典型的体内多聚磷酸盐体(PPB)含有氧(4.3×10⁻⁸微克)、碳(1.2×10⁻⁸微克)、磷(6.7×10⁻⁹微克)、镁(1.3×10⁻⁹微克)、钙(6.7×10⁻¹⁰微克)、钾(6.7×10⁻¹⁰微克)、铁(6.0×10⁻¹⁰微克)、硫(5.4×10⁻¹⁰微克)和铝(5.9×10⁻¹⁰微克)。对天然样品的定量X射线分析表明,PPB含有氧(1.63×10⁻⁸微克)、碳(4.75×10⁻⁹微克)、磷(2.50×10⁻⁹微克)、镁(5.0×10⁻¹⁰微克)、钙(2.50×10⁻¹⁰微克)、钾(2.50×10⁻¹⁰微克)、铁(2.25×10⁻¹⁰微克)和硫(2.0×10⁻¹⁰微克)。平均一个多聚磷酸盐体的质量,颤藻为6.7×10⁻⁸微克,金黄色葡萄球菌为2.5×10⁻⁸微克,天然环境中的微生物为6.3×10⁻⁸微克。结果表明,除了储存必需元素外,PPB可能还有其他未知功能,它可通过螯合重金属作为一种解毒方法,并在细胞中提供一个稳态系统。

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