Rima B K, Takahashi I
J Gen Microbiol. 1978 Jul;107(1):139-45. doi: 10.1099/00221287-107-1-139.
A number of deoxyribonucleoside-requiring mutants (dns) of Bacillus subtilis were isolated and their growth characteristics and ribonucleotide reductase activities were compared with those of the wild type and of a dna mutant (tsA13). Both tsA13 and dns mutants required the presence of a mixture of deoxyribonucleosides for growth at 45 degrees C but not at 25 degrees C. All the mutant strains tested contained ribonucleotide reductase activity which showed heat sensitivity similar to that of the enzyme from a wild-type strain. The reductase in B. subtilis seemed to reduce ribonucleoside triphosphates in a similar manner to the enzyme in Lactobacillus leichmannii.
分离出了许多枯草芽孢杆菌的脱氧核糖核苷需求突变体（dns），并将它们的生长特性和核糖核苷酸还原酶活性与野生型和一个DNA突变体（tsA13）进行了比较。tsA13和dns突变体在45℃生长时需要脱氧核糖核苷混合物的存在，但在25℃时则不需要。所有测试的突变菌株都含有核糖核苷酸还原酶活性，其热敏感性与野生型菌株的酶相似。枯草芽孢杆菌中的还原酶似乎以与leichmannii乳酸杆菌中的酶类似的方式还原三磷酸核糖核苷。
