Folmsbee Martha J, McInerney Michael J, Nagle David P
University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019-0245, USA.
Appl Environ Microbiol. 2004 Sep;70(9):5252-7. doi: 10.1128/AEM.70.9.5252-5257.2004.
Bacillus mojavensis strains JF-2 (ATCC 39307), ROB2, and ABO21191(T) and Bacillus subtilis strains 168 (ATCC 23857) and ATCC 12332 required four deoxyribonucleosides or DNA for growth under strict anaerobic conditions. Bacillus licheniformis strains L89-11 and L87-11, Bacillus sonorensis strain TG8-8, and Bacillus cereus (ATCC 14579) did not require DNA for anaerobic growth. The requirement for the deoxyribonucleosides or DNA did not occur under aerobic growth conditions. The addition of a mixture of five nucleic acid bases, four ribonucleotides, or four ribonucleosides to the basal medium did not replace the requirement of B. mojavensis JF-2 for the four deoxyribonucleosides. However, the addition of salmon sperm DNA, herring sperm DNA, Escherichia coli DNA, or synthetic DNA (single or double stranded) to the basal medium supported anaerobic growth. The addition of four deoxyribonucleosides to the basal medium allowed aerobic growth of B. mojavensis JF-2 in the presence of hydroxyurea. B. mojavensis did not grow in DNA-supplemented basal medium that lacked sucrose as the energy source. These data provide strong evidence that externally supplied deoxyribonucleosides can be used to maintain a balanced deoxyribonucleotide pool for DNA synthesis and suggest that ribonucleotide reductases may not be essential to the bacterial cell cycle nor are they necessarily part of a minimal bacterial genome.
莫哈韦芽孢杆菌菌株JF-2（ATCC 39307）、ROB2和ABO21191(T)以及枯草芽孢杆菌菌株168（ATCC 23857）和ATCC 12332在严格厌氧条件下生长需要四种脱氧核苷或DNA。地衣芽孢杆菌菌株L89-11和L87-11、索诺拉芽孢杆菌菌株TG8-8以及蜡样芽孢杆菌（ATCC 14579）在厌氧生长时不需要DNA。在有氧生长条件下不存在对脱氧核苷或DNA的需求。向基础培养基中添加五种核酸碱基、四种核糖核苷酸或四种核糖核苷的混合物并不能替代莫哈韦芽孢杆菌JF-2对四种脱氧核苷的需求。然而，向基础培养基中添加鲑鱼精DNA、鲱鱼精DNA、大肠杆菌DNA或合成DNA（单链或双链）能支持厌氧生长。向基础培养基中添加四种脱氧核苷能使莫哈韦芽孢杆菌JF-2在存在羟基脲的情况下进行有氧生长。莫哈韦芽孢杆菌在缺乏蔗糖作为能源的DNA补充基础培养基中无法生长。这些数据提供了有力证据，表明外部提供的脱氧核苷可用于维持用于DNA合成的平衡脱氧核苷酸库，并表明核糖核苷酸还原酶可能对细菌细胞周期并非必不可少，也不一定是最小细菌基因组的一部分。
