Mudron P, Rehage J, Qualmann K, Sallmann H P, Scholz H
Clinic of Internal Medicine (Ruminants and Swine), Veterinary University, Kosice, Slovakia.
Zentralbl Veterinarmed A. 1999 May;46(4):219-24. doi: 10.1046/j.1439-0442.1999.00206.x.
Sixteen dairy cows were studied to assess the status of the natural antioxidant vitamin E and lipid peroxidation in their livers. Cows with liver failure (n = 7) showed clinical signs of a hepatic encephalopathy and had the following values of selected blood indices: AST > 80 U/l and GLDH > 15 U/l in serum, and venous plasma ammonia > 35 mmol/l. The control group (n = 9) consisted of dairy cows which were recovering from surgery (omentopexy) and were free of any health complications. Blood was analysed for alpha-tocopherol, aspartate aminotransferase, glutamate dehydrogenase, gamma-glutamyl transferase, total bilirubin, ammonia, cholesterol, albumin, free fatty acids, glucose, and beta-hydroxybutyrate. Alpha-tocopherol, triglyceride and malondialdehyde were measured in wet liver tissue. The cows with hepatic failure were clearly low in alpha-tocopherol and had significantly lower (P < 0.01) plasma alpha-tocopherol than the controls. Both liver triglycerides and MDA were higher (P < 0.05) in the cows with fatty livers. It is concluded that the cows with liver failure had an increase in the intensity of hepatic lipoperoxidative processes and a low antioxidative status, which should be taken into consideration in cases where treatment of the disease is proposed.
对16头奶牛进行了研究，以评估其肝脏中天然抗氧化剂维生素E的状况和脂质过氧化情况。肝功能衰竭的奶牛（n = 7）表现出肝性脑病的临床症状，其选定血液指标的数值如下：血清中天冬氨酸转氨酶（AST）> 80 U/l，谷氨酸脱氢酶（GLDH）> 15 U/l，静脉血浆氨> 35 mmol/l。对照组（n = 9）由正在从手术（网膜固定术）中恢复且无任何健康并发症的奶牛组成。对血液进行了α-生育酚、天冬氨酸转氨酶、谷氨酸脱氢酶、γ-谷氨酰转移酶、总胆红素、氨、胆固醇、白蛋白、游离脂肪酸、葡萄糖和β-羟基丁酸的分析。在湿肝组织中测量了α-生育酚、甘油三酯和丙二醛。肝功能衰竭的奶牛肝脏中的α-生育酚明显偏低，血浆α-生育酚显著低于对照组（P < 0.01）。脂肪肝奶牛的肝脏甘油三酯和丙二醛均较高（P < 0.05）。得出的结论是，肝功能衰竭的奶牛肝脏脂质过氧化过程的强度增加且抗氧化状态较低，在提出疾病治疗方案时应考虑到这一点。
