Haddaoui E, Chambert R, Petit-Glatron M F, Lindy O, Sarvas M
Institut Jacques Monod-C.N.R.S, Universités Paris, France.
FEMS Microbiol Lett. 1999 Apr 1;173(1):127-31. doi: 10.1111/j.1574-6968.1999.tb13493.x.
When Bacillus subtilis alpha-amylase was expressed under the control of sacR in a degU32(Hy) strain, the production of exoenzyme occurred during both the exponential and stationary phases of growth. In each phase, pulse-chase experiments showed that the rate-limiting step of the secretion process was the release of the processed form of the protein in each physiological context. The rate of this event was slightly slower (t(1/2) = 3.2 min) during the stationary phase than during the exponential phase (t(1/2) = 2 min). The effectors which possibly control the efficiency of the release stage, the level of PrsA or the calcium binding properties of the cell wall, remained unchanged throughout growth phases.
