不动杆菌属ADP1菌株中参与烷烃降解的rubA和rubB基因与编码酯酶的estB以及oxyR处于同一个操纵子中。
The genes rubA and rubB for alkane degradation in Acinetobacter sp. strain ADP1 are in an operon with estB, encoding an esterase, and oxyR.
作者信息
Geissdörfer W, Kok R G, Ratajczak A, Hellingwerf K J, Hillen W
机构信息
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany.
出版信息
J Bacteriol. 1999 Jul;181(14):4292-8. doi: 10.1128/JB.181.14.4292-4298.1999.
Abstract
Alkanes are oxidized in Acinetobacter sp. strain ADP1 by a three-component alkane monooxygenase, composed of alkane hydroxylase, rubredoxin, and rubredoxin reductase. rubA and rubB encode rubredoxin and a NAD(P)H-dependent rubredoxin reductase. We demonstrate here that single base pair substitutions in rubA or rubB lead to defects in alkane degradation, showing that both genes are essential for alkane utilization. Differences in the degradation capacity for hexadecane and dodecane in these mutants are discussed. Two genes, estB and oxyR, are located downstream of rubB, but are not necessary for alkane degradation. estB encodes a functional esterase. oxyR encodes a LysR-type transcriptional regulator, conferring resistance to hydrogen peroxide. rubA, rubB, estB, and oxyR constitute an operon, which is constitutively transcribed from a sigma70 promoter, and an estB-oxyR containing message is also transcribed from an internal promoter.
摘要
在不动杆菌属ADP1菌株中，烷烃由一种三组分烷烃单加氧酶氧化，该酶由烷烃羟化酶、红素氧还蛋白和红素氧还蛋白还原酶组成。rubA和rubB分别编码红素氧还蛋白和一种依赖NAD(P)H的红素氧还蛋白还原酶。我们在此证明，rubA或rubB中的单碱基对替换会导致烷烃降解缺陷，表明这两个基因对于烷烃利用都是必不可少的。讨论了这些突变体中十六烷和十二烷降解能力的差异。estB和oxyR这两个基因位于rubB的下游，但对于烷烃降解并非必需。estB编码一种功能性酯酶。oxyR编码一种LysR型转录调节因子，赋予对过氧化氢的抗性。rubA、rubB、estB和oxyR构成一个操纵子，该操纵子从σ70启动子组成型转录，并且一个包含estB-oxyR的信使也从内部启动子转录。
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