构建并分析黄单胞菌突变体以研究oxyR基因在氧化剂诱导的抗过氧化物杀伤保护作用中的角色。
Construction and physiological analysis of a Xanthomonas mutant to examine the role of the oxyR gene in oxidant-induced protection against peroxide killing.
作者信息
Mongkolsuk S, Sukchawalit R, Loprasert S, Praituan W, Upaichit A
机构信息
Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210, Thailand.
出版信息
J Bacteriol. 1998 Aug;180(15):3988-91. doi: 10.1128/JB.180.15.3988-3991.1998.
Abstract
We constructed and characterized a Xanthomonas campestris pv. phaseoli oxyR mutant. The mutant was hypersensitive to H2O2 and menadione killing and had reduced aerobic plating efficiency. The oxidants' induction of the catalase and ahpC genes was also abolished in the mutant. Analysis of the adaptive responses showed that hydrogen peroxide-induced protection against hydrogen peroxide was lost, while menadione-induced protection against hydrogen peroxide was retained in the oxyR mutant. These results show that X. campestris pv. phaseoli oxyR is essential to peroxide adaptation and revealed the existence of a novel superoxide-inducible peroxide protection system that is independent of OxyR.
摘要
我们构建并鉴定了菜豆黄单胞菌菜豆致病变种的oxyR突变体。该突变体对过氧化氢和甲萘醌杀伤高度敏感，需氧平板培养效率降低。在该突变体中，氧化剂对过氧化氢酶和ahpC基因的诱导作用也被消除。适应性反应分析表明，过氧化氢诱导的对过氧化氢的保护作用在oxyR突变体中丧失，而甲萘醌诱导的对过氧化氢的保护作用在该突变体中得以保留。这些结果表明，菜豆黄单胞菌菜豆致病变种的oxyR对于过氧化物适应至关重要，并揭示了一种独立于OxyR的新型超氧化物诱导的过氧化物保护系统的存在。
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