Bromage E S, Thomas A, Owens L
Department of Microbiology and Immunology, James Cook University, Townsville, Queensland, Australia.
Dis Aquat Organ. 1999 May 31;36(3):177-81. doi: 10.3354/dao036177.
The cause of ongoing mortality in barramundi Lates calcarifer (Bloch) in seawater culture was identified as Streptococcus iniae by biochemical and physiological tests. This is the first published record of this bacterial species in Australia and the first confirmed report of S. iniae causing mortality in barramundi. The bacterium was highly pathogenic for barramundi when challenged by bath exposure. The pathogen was found to have a LD50 of 2.5 x 10(5) and 3.2 x 10(4) colony-forming units at 48 h and 10 d respectively. Experimental challenge of barramundi resulted in high levels of mortality (> 40%) within a 48 h period. Ten days after the challenge, S. iniae could not be isolated from kidney, spleen, liver or eye of surviving fish. However, the organism was easily isolated from the brain of both moribund and healthy fish, indicating that barramundi can carry the bacterium asymptomatically.
通过生化和生理学测试,确定海水养殖的尖吻鲈(Lates calcarifer,布洛赫)持续死亡的原因是海豚链球菌。这是该细菌物种在澳大利亚首次发表的记录,也是海豚链球菌导致尖吻鲈死亡的首次确诊报告。当通过浸浴暴露进行攻毒时,该细菌对尖吻鲈具有高度致病性。发现该病原体在48小时和10天时的半数致死剂量分别为2.5×10⁵和3.2×10⁴菌落形成单位。对尖吻鲈进行实验性攻毒导致在48小时内出现高水平的死亡率(>40%)。攻毒十天后,在存活鱼的肾脏、脾脏、肝脏或眼睛中无法分离出海豚链球菌。然而,该生物体很容易从濒死和健康鱼的大脑中分离出来,这表明尖吻鲈可以无症状携带该细菌。