Ema M, Miyawaki E, Kawashima K
National Institute of Health Sciences, Osaka Branch, Japan.
Arch Toxicol. 1999 Apr-May;73(3):175-9. doi: 10.1007/s002040050603.
In our previous study, triphenyltin chloride (TPTC1) was found to induce implantation failure, as preimplantation embryonic loss, in rats. In this study, the effects of TPTC1 on the uterine function, as a cause of implantation failure, were determined using pseudopregnant rats. Female rats were given TPTC1 by gastric intubation at 3.1, 4.7, and 6.3 mg/kg on pseudopregnant day (PPD) 0 to PPD 3 and the decidual cell response was induced on PPD 4. The uterine weight on PPD 9 served as an index of uterine decidualization. A significant decrease in the uterine weight, which indicates suppression of the uterine decidualization, was detected at 4.7 and 6.3 mg/kg. In our previous study, these doses induced a significant increase in implantation failure in female rats given TPTC1 on gestational day (GD) 0 to 3. The ovarian weight and number of corpora lutea in the TPTC1-treated groups were comparable to that of the controls. A significant decrease in serum progesterone levels after administration of TPTC1 was found at 4.7 and 6.3 mg/kg. These findings suggest that implantation failure due to TPTC1 may be mediated via the suppression of uterine decidualization and correlated with the reduction in serum progesterone levels.
在我们之前的研究中，发现三苯基氯化锡（TPTC1）会导致大鼠出现着床失败，即着床前胚胎丢失。在本研究中，使用假孕大鼠确定了TPTC1对子宫功能（作为着床失败的一个原因）的影响。在假孕第（PPD）0至PPD 3天，给雌性大鼠经胃插管给予3.1、4.7和6.3 mg/kg的TPTC1，并在PPD 4天诱导蜕膜细胞反应。PPD 9天的子宫重量作为子宫蜕膜化的指标。在4.7和6.3 mg/kg剂量下，检测到子宫重量显著下降，这表明子宫蜕膜化受到抑制。在我们之前的研究中，这些剂量在妊娠第（GD）0至3天给予TPTC1的雌性大鼠中诱导着床失败显著增加。TPTC1处理组的卵巢重量和黄体数量与对照组相当。在4.7和6.3 mg/kg剂量下，发现给予TPTC1后血清孕酮水平显著下降。这些发现表明，TPTC1导致的着床失败可能是通过抑制子宫蜕膜化介导的，并且与血清孕酮水平降低相关。
