Harazono A, Ema M
National Institute of Health Sciences, Osaka Branch, Japan.
Arch Toxicol. 2000 Dec;74(10):632-7. doi: 10.1007/s002040000163.
In our previous studies, tributyltin chloride (TBTCl) at doses of 16.3 mg/kg and above caused implantation failure (preimplantation embryonic loss) and postimplantation embryonic loss in rats following administration on gestational day (GD) 0 through GD 3 and GD 4 through GD 7, respectively. This study was designed to assess the effects of TBTCl on uterine function as a cause of early embryonic loss in pseudopregnant rats. TBTCl was given orally to pseudopregnant rats at doses of 4.1, 8.1, 16.3 and 32.5 mg/kg on pseudopregnant day (PPD) 0 to PPD 3 or 8.1, 16.3, 32.5 and 65.1 mg/kg on PPD 4 to PPD 7. The decidual cell response was induced by bilateral scratch trauma on PPD 4. The uterine weight on PPD 9 served as an index of uterine decidualization. Uterine weight and serum progesterone levels on PPD 9 were significantly decreased after administration of TBTCl at doses of 16.3 mg/kg and above on PPD 0 to PPD 3 or PPD 4 to PPD 7. Administration of TBTCl at doses of 8.1 mg/kg and above on PPD 0 to 3 also significantly decreased serum progesterone levels on PPD 4. TBTCl had no effect on ovarian weight and number of corpora lutea. It can be concluded that TBTCl suppresses the uterine decidual cell response and decreases progesterone levels, and these effects are responsible for early embryonic loss due to TBTCl exposure.
在我们之前的研究中，氯化三丁基锡（TBTCl）剂量在16.3毫克/千克及以上时，分别在妊娠第0天至第3天以及第4天至第7天给药后，会导致大鼠着床失败（着床前胚胎丢失）和着床后胚胎丢失。本研究旨在评估TBTCl对子宫功能的影响，将其作为假孕大鼠早期胚胎丢失的一个原因。在假孕第0天至第3天，以4.1、8.1、16.3和32.5毫克/千克的剂量给假孕大鼠口服TBTCl；在假孕第4天至第7天，以8.1、16.3、32.5和65.1毫克/千克的剂量给药。在假孕第4天通过双侧划痕创伤诱导蜕膜细胞反应。假孕第9天的子宫重量作为子宫蜕膜化的指标。在假孕第0天至第3天或第4天至第7天给予16.3毫克/千克及以上剂量的TBTCl后，假孕第9天的子宫重量和血清孕酮水平显著降低。在假孕第0天至第3天给予8.1毫克/千克及以上剂量的TBTCl也显著降低了假孕第4天的血清孕酮水平。TBTCl对卵巢重量和黄体数量没有影响。可以得出结论，TBTCl抑制子宫蜕膜细胞反应并降低孕酮水平，这些影响是TBTCl暴露导致早期胚胎丢失的原因。
