Williams R B
Wellcome Research Laboratories, Berkhamsted, Hertfordshire, UK.
Vet Res Commun. 1999 May;23(3):151-63. doi: 10.1023/a:1006273500290.
Nine enzymes were detected in sporulated oocysts of Eimeria tenella and E. maxima, parasites of the domesticated fowl (Gallus gallus). Three enzymes, hydroxybutyrate dehydrogenase, alanine aminotransferase and gamma-glutamyltransferase, all identified for the first time in Eimeria of fowl, occurred both in E. tenella and in E. maxima. The remaining enzymes assayed had previously been found in various Eimeria species of fowl, although creatine kinase and glutamate dehydrogenase were hitherto unknown from E. maxima. The three enzymes newly recorded from Eimeria of fowl are of interest as potential genetic markers, and also as potential chemotherapeutic targets. The discovery of hydroxybutyrate dehydrogenase led to the demonstration of anticoccidial activity by some aliphatic acids. The paper also includes a list of the enzymes detected in Eimeria of fowl in previous studies.
在家禽（原鸡）的寄生虫柔嫩艾美耳球虫和巨型艾美耳球虫的孢子化卵囊中检测到了九种酶。三种酶，即羟丁酸脱氢酶、丙氨酸转氨酶和γ-谷氨酰转移酶，均首次在家禽艾美耳球虫中被鉴定出来，它们同时存在于柔嫩艾美耳球虫和巨型艾美耳球虫中。其余检测的酶此前已在家禽的各种艾美耳球虫物种中发现，不过肌酸激酶和谷氨酸脱氢酶在巨型艾美耳球虫中迄今尚属未知。在家禽艾美耳球虫中新记录的这三种酶作为潜在的遗传标记以及潜在的化疗靶点很受关注。羟丁酸脱氢酶的发现导致了一些脂肪酸抗球虫活性的证明。本文还列出了此前研究在家禽艾美耳球虫中检测到的酶的清单。
