Wakabayashi I
Department of Hygiene and Preventive Medicine, School of Medicine, Yamagata University, Japan.
Pharmacol Toxicol. 1999 Jun;84(6):288-91. doi: 10.1111/j.1600-0773.1999.tb01496.x.
In order to elucidate the mechanism of the antiinflammatory action of baicalein and wogonin, flavonoids from the root of Scutellaria baicalensis, the effects of these compounds were investigated on lipopolysaccharide-induced nitric oxide production in a macrophage-derived cell line, RAW 264.7. Baicalein (5-25 microM) and wogonin (5-50 microM) inhibited lipopolysaccharide-induced nitric oxide generation in a concentration-dependent manner. The inhibitory effect of these compounds was observed only when they were added at the start of cell incubation soon after the stimulation with lipopolysaccharide. Baicalein (25 microM) and wogonin (25 microM) also inhibited protein expression of inducible nitric oxide synthase. This inhibitory effect of wogonin was stronger than that of baicalein, which agrees with the result that wogonin showed stronger inhibition of nitric oxide production than baicalein. These results suggest that baicalein and wogonin attenuate lipopolysaccharide-stimulated nitric oxide synthase induction in macrophages and thus may help to explain the antiinflammatory action of these flavonoid compounds.
为阐明黄芩根中的黄酮类化合物黄芩素和汉黄芩素的抗炎作用机制，研究了这些化合物对巨噬细胞系RAW 264.7中脂多糖诱导的一氧化氮生成的影响。黄芩素（5 - 25微摩尔）和汉黄芩素（5 - 50微摩尔）以浓度依赖的方式抑制脂多糖诱导的一氧化氮生成。仅当在脂多糖刺激后细胞孵育开始时添加这些化合物时，才观察到它们的抑制作用。黄芩素（25微摩尔）和汉黄芩素（25微摩尔）也抑制诱导型一氧化氮合酶的蛋白表达。汉黄芩素的这种抑制作用比黄芩素更强，这与汉黄芩素对一氧化氮生成的抑制作用比黄芩素更强的结果一致。这些结果表明，黄芩素和汉黄芩素减弱了巨噬细胞中脂多糖刺激的一氧化氮合酶诱导，因此可能有助于解释这些黄酮类化合物的抗炎作用。
