Buchman S R, Ozaki W
Department of Surgery, University of Michigan Medical Center, Ann Arbor, USA.
Ann Plast Surg. 1999 Jul;43(1):49-56. doi: 10.1097/00000637-199907000-00008.
The authors' laboratory has shown cancellous onlay bone grafts to resorb faster than cortical grafts. To understand the nature of cancellous bone grafts beyond volumetric measurements, a temporal analysis of the internal microarchitecture of these grafts was performed. Their hypothesis is that the forces of remodeling and resorption cause cancellous onlay bone grafts to develop a denser, more interconnected, and a more mechanically stable microarchitecture. Twenty-five adult New Zealand White rabbits were used in this study and were divided into three groups. Microcomputed tomography (MCT) was performed on all cancellous bone grafts to obtain detailed information regarding the microarchitecture of the cancellous bone. Bone graft specimens were examined histologically, and histomorphometric analysis was also performed. Their results show that cancellous onlay bone grafts develop a higher bone volume fraction, mean trabecular thickness, connectivity, and degree of anisotropy. Furthermore, cancellous onlay bone grafts developed a lower bone surface area-to-volume ratio and mean trabecular separation. The unique combination of MCT technology and histomorphometric techniques proved to be effective in providing a qualitative and quantitative ultrastructural analysis of cancellous onlay bone grafts over time. The authors were able to show changes in the internal microarchitecture of cancellous onlay bone grafts as they were remodeled and resorbed. Specifically, they found the cancellous onlay bone grafts to develop a more dense, less trabecular, more organized, and more interconnected internal ultrastructure over time. Their findings have helped to provide a reproducible description of the temporal sequence of changes in bone microarchitecture, revascularization, and internal remodeling.
作者所在实验室已表明，松质骨覆盖移植骨的吸收速度比皮质骨移植骨更快。为了在体积测量之外了解松质骨移植骨的本质，对这些移植骨的内部微观结构进行了时间分析。他们的假设是，重塑和吸收的力量会使松质骨覆盖移植骨形成更致密、相互连接性更强且机械稳定性更高的微观结构。本研究使用了25只成年新西兰白兔，并将其分为三组。对所有松质骨移植骨进行了微型计算机断层扫描（MCT），以获取有关松质骨微观结构的详细信息。对骨移植标本进行了组织学检查，并进行了组织形态计量分析。他们的结果表明，松质骨覆盖移植骨形成了更高的骨体积分数、平均小梁厚度、连通性和各向异性程度。此外，松质骨覆盖移植骨的骨表面积与体积比和平均小梁间距更低。事实证明，MCT技术和组织形态计量技术的独特结合能够有效地对松质骨覆盖移植骨随时间变化进行定性和定量的超微结构分析。作者能够展示松质骨覆盖移植骨在重塑和吸收过程中内部微观结构的变化。具体而言，他们发现随着时间的推移，松质骨覆盖移植骨会形成更致密、小梁更少、更有组织且相互连接性更强的内部超微结构。他们的研究结果有助于对骨微观结构、血管再生和内部重塑变化的时间顺序提供可重复的描述。
