Simard F, Fontenille D, Lehmann T, Girod R, Brutus L, Gopaul R, Dournon C, Collins F H
Laboratoire de Zoologic Médicale, French Institute de Recherche pour le Développement, Institut Pasteur, Dakar, Senegal.
Am J Trop Med Hyg. 1999 Jun;60(6):1000-9. doi: 10.4269/ajtmh.1999.60.1000.
Polymorphism at nine microsatellite loci was examined to assess the level of genetic differentiation between four Anopheles arabiensis populations from Senegal, the high plateau of Madagascar, and Reunion and Mauritius islands. Eight of nine loci showed great polymorphism (2-16 alleles/locus) and significant genetic differentiation was revealed between all four populations by F- and R-statistics, with Fst estimates ranging from 0.080 to 0.215 and equivalent Rst values ranging between 0.022 and 0.300. These high amounts of genetic differentiation are discussed in relation to geographic distance including large bodies of water, and history of mosquito settlement, and insecticide use on the islands. The results suggest that historical events of drift rather than mutation are probably the forces generating genetic divergence between these populations, with homogenization of the gene pool by migration being drastically restricted across the ocean.
对九个微卫星位点的多态性进行了检测，以评估来自塞内加尔、马达加斯加高原以及留尼汪岛和毛里求斯岛的四个阿拉伯按蚊种群之间的遗传分化水平。九个位点中的八个显示出高度多态性（每个位点有2 - 16个等位基因），通过F统计量和R统计量揭示了所有四个种群之间存在显著的遗传分化，Fst估计值范围为0.080至0.215，等效的Rst值范围在0.022至0.300之间。结合包括大片水域在内的地理距离、蚊子定居历史以及岛屿上的杀虫剂使用情况，对这些高度的遗传分化进行了讨论。结果表明，导致这些种群之间遗传分歧的力量可能是漂变的历史事件而非突变，跨越大洋的迁移对基因库的同质化产生了极大限制。
