Noakes K L, Teisserenc H T, Lord J M, Dunbar P R, Cerundolo V, Roberts L M
Department of Biological Sciences, University of Warwick, Coventry, UK.
FEBS Lett. 1999 Jun 18;453(1-2):95-9. doi: 10.1016/s0014-5793(99)00609-2.
Shiga-like toxin 1 (SLT) from Escherichia coli O157:H7 enters mammalian cells by endocytosis from the cell surface to the endoplasmic reticulum before translocating into the cytosol. Here, SLT was engineered at its N- or C-terminus to carry a peptide derived from influenza virus Matrix protein for delivery to major histocompatibility complex (MHC) class I molecules. We show that SLT N-Ma was capable of sensitising cells for lysis by appropriate cytotoxic T-lymphocytes whilst no killing of SLT-resistant cells was observed. Our results demonstrate that peptide was liberated intracellularly and that retrograde transport of a disarmed cytotoxic protein can intersect the MHC class 1 presentation pathway.
来自大肠杆菌O157:H7的志贺样毒素1（SLT）通过内吞作用从细胞表面进入哺乳动物细胞，直至内质网，然后转移至细胞质中。在此，我们对SLT的N端或C端进行改造，使其携带一段源自流感病毒基质蛋白的肽段，以便递送至主要组织相容性复合体（MHC）I类分子。我们发现，SLT N-Ma能够使细胞对相应的细胞毒性T淋巴细胞的裂解作用敏感，而对SLT抗性细胞则未观察到杀伤作用。我们的结果表明，肽段在细胞内被释放，并且一种失活的细胞毒性蛋白的逆向转运能够与MHC I类呈递途径相交。
