利用免疫吸附电子显微镜对蓝舌病毒重组核心样颗粒进行定量分析。

Zheng Y Z, Hyatt A, Wang L F, Eaton B T, Greenfield P F, Reid S

Department of Chemical Engineering, The University of Queensland, Australia.

J Virol Methods. 1999 Jun;80(1):1-9. doi: 10.1016/s0166-0934(98)00170-0.

Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. The capture antibody was an anti-BTV VP7 monoclonal antibody. The CLP concentration in purified preparations was determined to be 6.6 x 10(15) particles/l. CLP concentration in lysates of recombinant baculovirus-infected cells was determined at various times post-infection and shown to reach a value of 3 x 10(15) particles/l of culture medium at 96 h post-infection. The results indicated that immunosorbent electron microscopy, aided by an improved particle counting method, is a simple, rapid and accurate technique for the quantification of virus and virus-like particles produced in large scale in vitro systems.

免疫吸附电子显微镜技术用于定量纯化制剂或重组杆状病毒感染细胞裂解物中重组杆状病毒产生的蓝舌病病毒（BTV）核心样颗粒（CLP）。捕获抗体为抗BTV VP7单克隆抗体。纯化制剂中的CLP浓度测定为6.6×10¹⁵个颗粒/升。在感染后不同时间测定重组杆状病毒感染细胞裂解物中的CLP浓度，结果显示在感染后96小时达到3×10¹⁵个颗粒/升培养基的值。结果表明，借助改进的颗粒计数方法，免疫吸附电子显微镜技术是一种用于定量大规模体外系统中产生的病毒和病毒样颗粒的简单、快速且准确的技术。