Marshall J J, Fayard B, Roy P
NERC Institute of Virology and Environmental Microbiology, Oxford, U.K.
J Gen Virol. 1990 Aug;71 ( Pt 8):1839-44. doi: 10.1099/0022-1317-71-8-1839.
Bluetongue virus tubules were purified from Spodoptera frugiperda cells infected with a recombinant baculovirus containing the NS1 gene from bluetongue virus serotype 10, and expressed under control of the Autographa californica nuclear polyhedrosis virus polyhedrin promoter. These tubules were subjected to a variety of chemical and physical treatments and the resulting effects on tubule morphology were examined by electron microscopy. A number of morphological similarities were noted between bluetongue virus tubules and cellular microtubules despite a lack of homology between the component proteins at the primary sequence level. A possible multistranded helical configuration is proposed for the tubule structure.
从感染了含有蓝舌病毒10型NS1基因的重组杆状病毒的草地贪夜蛾细胞中纯化出蓝舌病毒小管，其在苜蓿银纹夜蛾核型多角体病毒多角体蛋白启动子的控制下表达。对这些小管进行了各种化学和物理处理，并通过电子显微镜检查了对小管形态产生的影响。尽管组成蛋白在一级序列水平上缺乏同源性，但在蓝舌病毒小管和细胞微管之间仍观察到许多形态学上的相似之处。提出了一种可能的多链螺旋结构用于小管结构。
