Huby F, Nano J L, Mallet S, Hoste H
INRA, Station de Pathologie Aviaire et de Parasitologie, CR de Tours, Nouzilly, France.
Int J Parasitol. 1999 May;29(5):697-702. doi: 10.1016/s0020-7519(99)00014-4.
The effects of the excretory/secretory (ES) products of the parasitic nematode Trichostrongylus colubriformis were examined on the proliferation of seven cell lines derived from a digestive or non-digestive origin. The excretory/secretory products of T. colubriformis were incorporated in the culture medium of the different cell lines and cell proliferation was measured by means of the 5-bromo-2'-deoxy-uridine (Brdu) assay. An increase in cell numbers was found with the three epithelial intestinal cells (RIC, IEC-6, IRD-98) and with epithelial kidney cells (MDCK). In contrast, an inhibition in the proliferation of epithelial ovarian cells (CHO) and fibroblasts (3T3) was observed with the addition of the excretory/secretory products and no effect was detected on the cell growth of hepatocytes (HepG2). These data are discussed with respect to the tissue specificity of the existing mitogenic effect of the worms on the intestinal crypt cells during parasitism.
研究了寄生线虫蛇形毛圆线虫排泄/分泌（ES）产物对源自消化或非消化器官的七种细胞系增殖的影响。将蛇形毛圆线虫的排泄/分泌产物加入不同细胞系的培养基中，并通过5-溴-2'-脱氧尿苷（Brdu）测定法测量细胞增殖。发现三种肠上皮细胞（RIC、IEC-6、IRD-98）和肾上皮细胞（MDCK）的细胞数量增加。相反，添加排泄/分泌产物后，观察到上皮卵巢细胞（CHO）和成纤维细胞（3T3）的增殖受到抑制，并且未检测到对肝细胞（HepG2）细胞生长的影响。结合寄生虫感染期间蠕虫对肠道隐窝细胞现有促有丝分裂作用的组织特异性对这些数据进行了讨论。
