体细胞抗原对K562细胞生长抑制的抗肿瘤作用
Anti-Tumor Effect of Somatic Antigen on Inhibition Cell Growth of K562.
作者信息
Raisnia Reyhane, Mohebalian Hadi, Shahrokhi Sabzevar Atousa, Borji Hassan
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
出版信息
Iran J Parasitol. 2022 Jan-Mar;17(1):28-35. doi: 10.18502/ijpa.v17i1.9013.
BACKGROUND
Today, the inhibitory effect of helminths on tumor cell growth has been proven. We investigated the anti-tumor activity of somatic against K562 cells.
METHODS
Different concentrations of somatic antigen were incorporated in the culture medium of K652 cells, and the proliferation and apoptosis were measured after 24, 48, and 72 h, using MTT and Annexin V/PI staining assay.
RESULTS
Treatment of cells with 1-2 mg/ml antigen for 24-72 h could suppress cell proliferation and increase apoptosis. While treating cells with 0.1 mg/ml antigen for 72 h could inhibit cell growth. There was no meaningful effect on treated cells in comparison with the control group (< 0.05) after incubating cells for 24- 48 h with 0.1 mg/ml antigen.
CONCLUSION
somatic antigen had an anti-cancer property, and its role in cancer treatment could be considered as an effective therapeutic method.
背景
如今，蠕虫对肿瘤细胞生长的抑制作用已得到证实。我们研究了[虫体名称未明确，推测为somatic指代的某种虫体]抗原对K562细胞的抗肿瘤活性。
方法
将不同浓度的[虫体名称未明确，推测为somatic指代的某种虫体]抗原加入K652细胞的培养基中，在24、48和72小时后，使用MTT和Annexin V/PI染色法检测细胞增殖和凋亡情况。
结果
用1 - 2毫克/毫升抗原处理细胞24 - 72小时可抑制细胞增殖并增加凋亡。而用0.1毫克/毫升抗原处理细胞72小时可抑制细胞生长。用0.1毫克/毫升抗原处理细胞24 - 48小时后，与对照组相比，对处理后的细胞没有显著影响（<0.05）。
结论
[虫体名称未明确，推测为somatic指代的某种虫体]抗原具有抗癌特性，其在癌症治疗中的作用可被视为一种有效的治疗方法。
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